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dc.contributor.author
Cerda, Maria Belen  
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Batalla, Milena  
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Anton, Martina  
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Cafferata, Eduardo Gustavo Alfredo  
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Podhajcer, Osvaldo Luis  
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Plank, Christian  
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Mykhaylyk, Olga  
dc.contributor.author
Policastro, Lucia Laura  
dc.date.available
2017-03-14T14:22:38Z  
dc.date.issued
2015  
dc.identifier.citation
Cerda, Maria Belen; Batalla, Milena; Anton, Martina; Cafferata, Eduardo Gustavo Alfredo; Podhajcer, Osvaldo Luis; et al.; Enhancement of nucleic acid delivery to hard-to-transfect human colorectal cancer cells by magnetofection at laminin coated substrates and promotion of the endosomal/lysosomal escape; Royal Society of Chemistry; RSC Advances; 72; 5; 2015; 58345-58354  
dc.identifier.issn
2046-2069  
dc.identifier.uri
http://hdl.handle.net/11336/13821  
dc.description.abstract
Despite a great diversity of nanomaterials, such as cationic lipid, polymers or inorganic nanoparticles, has been developed in order to carry nucleic acids across plasma membrane, these methodologies have still insufficient efficacy in cells named hard-to-transfect cells, as the colorectal HT29 and Caco-2 cell lines. This paper describes the improvement of plasmid DNA (pDNA) and small interfering RNA (siRNA) transfer in these cells through the combination of magnetofection, a simplified extracellular matrix of laminin and endosomal/lysosomal escape promotion using the endosome-disruptive peptide INF-7. Magnetofection of pDNA complexes using selected vector formulations resulted in up to 2-fold enhancement in luciferase expression, as compared to lipofection. Further enhance in pDNA transfer in HT29 cells was obtained when magnetofection was applied on cells grown on laminin coated substrates, increasing 6-fold the luciferase expression compared to lipofection at uncoated substrates. This technique was also applied to siRNA delivery in cells expressing stably luciferase (Caco-2Luc and HT29Luc), selected magnetic vector formulations resulted in 61±5% and 50±5% of luciferase silencing in HT29Luc and Caco-2Luc, respectively. Further improvement in reporter gene silencing was obtained when the magnetic complexes were modified with INF-7, reaching more than 95% of luciferase silencing in Caco-2Luc cells, while pre-treatment of HT29Luc cells by chloroquine resulted in 80±4% of down regulation of luciferase expression. Thus, magnetofection applied on cells grown over laminin coated substrates and the optimization of endosomal escape of magnetic complexes would be a good alternative to enhance nucleic acid transfer in hard-to-transfect colorectal cancer cells.  
dc.format
application/pdf  
dc.language.iso
eng  
dc.publisher
Royal Society of Chemistry  
dc.rights
info:eu-repo/semantics/openAccess  
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/  
dc.subject
Magnetic Nanoparticles  
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Magnetofection  
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Laminin  
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Inf-7  
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Otras Nanotecnología  
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Nanotecnología  
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INGENIERÍAS Y TECNOLOGÍAS  
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Otros Tópicos Biológicos  
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Ciencias Biológicas  
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CIENCIAS NATURALES Y EXACTAS  
dc.title
Enhancement of nucleic acid delivery to hard-to-transfect human colorectal cancer cells by magnetofection at laminin coated substrates and promotion of the endosomal/lysosomal escape  
dc.type
info:eu-repo/semantics/article  
dc.type
info:ar-repo/semantics/artículo  
dc.type
info:eu-repo/semantics/publishedVersion  
dc.date.updated
2016-12-16T17:26:39Z  
dc.journal.volume
72  
dc.journal.number
5  
dc.journal.pagination
58345-58354  
dc.journal.pais
Reino Unido  
dc.journal.ciudad
Londres  
dc.description.fil
Fil: Cerda, Maria Belen. Comision Nacional de Energia Atomica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto de Nanociencias y Nanotecnología; Argentina  
dc.description.fil
Fil: Batalla, Milena. Comision Nacional de Energia Atomica; Argentina. Instituto de Nanociencias y Nanotecnología; Argentina  
dc.description.fil
Fil: Anton, Martina. Technische Universität München; Alemania  
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Fil: Cafferata, Eduardo Gustavo Alfredo. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquimicas de Buenos Aires; Argentina. Fundación Instituto Leloir; Argentina  
dc.description.fil
Fil: Podhajcer, Osvaldo Luis. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquimicas de Buenos Aires; Argentina. Fundación Instituto Leloir; Argentina  
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Fil: Plank, Christian. Technische Universität München; Alemania  
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Fil: Mykhaylyk, Olga. Technische Universität München; Alemania  
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Fil: Policastro, Lucia Laura. Comision Nacional de Energia Atomica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto de Nanociencias y Nanotecnología; Argentina  
dc.journal.title
RSC Advances  
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/http://pubs.rsc.org/en/content/articlelanding/2015/ra/c5ra06562c#!divAbstract  
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/http://dx.doi.org/10.1039/C5RA06562C