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dc.contributor.author
Temprana, Carlos Facundo
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dc.contributor.author
Amor, M. Silvia
dc.contributor.author
Femia, Lis
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dc.contributor.author
Gasparri, Julieta
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dc.contributor.author
Taira, M. Cristina
dc.contributor.author
Alonso, Silvia del Valle
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dc.date.available
2017-03-14T13:56:03Z
dc.date.issued
2011-06
dc.identifier.citation
Temprana, Carlos Facundo; Amor, M. Silvia; Femia, Lis; Gasparri, Julieta; Taira, M. Cristina; et al.; Ultraviolet irradiation of diacetylenic liposomes as a strategy to improve size stability and to alter protein binding without cytotoxicity enhancement; Taylor & Francis; Journal Of Liposome Research; 21; 2; 6-2011; 141-150
dc.identifier.issn
0898-2104
dc.identifier.uri
http://hdl.handle.net/11336/13818
dc.description.abstract
Membrane-modification effects, induced by ultraviolet (UV) irradiation in diacetylenic liposomes, were analyzed upon contact with cells, biological membranes, and proteins. Liposomes formulated with mixtures of unsaturated 1,2-bis(10,12-tricosadiynoyl)-sn-glycero-3-phosphocholine and saturated 1,2-dimyristoyl-sn-glycero-3-phosphocholine, in a 1:1 molar ratio, were compared with those that were UV-irradiated and analyzed in several aspects. Membrane polymerization inherence on size stability was studied as well as its impact on mitochondrial and microsomal membrane peroxidation induction, hemolytic activity, and cell viability. Moreover, in order to gain insight about the possible irradiation effect on interfacial membrane properties, interaction with bovine serum albumin (BSA), lysozyme (Lyso), and apolipoprotein (apoA-I) was studied. Improved size stability was found for polymerized liposomes after a period of 30 days at 4°C. In addition, membrane irradiation had no marked effect on cell viability, hemolysis, or induction of microsomal and mitochondrial membrane peroxidation. Interfacial membrane characteristics were found to be altered after polymerization, since a differential protein binding for polymerized or nonpolymerized membranes was observed for BSA and Lyso, but not for apoA-I. The substantial contribution of this work is the finding that even when maintaining the same lipid composition, changes induced by UV irradiation are sufficient to increase size stability and establish differences in protein binding, in particular, reducing the amount of bound Lyso and BSA, without increasing formulation cytotoxicity. This work aimed at showing that the usage of diacetylenic lipids and UV modification of membrane interfacial properties should be strategies to be taken into consideration when designing new delivery systems.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Taylor & Francis
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dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
Diacetylenic Lipids
dc.subject
Lipid Peroxidation
dc.subject
Drug Delivery
dc.subject
Cytotoxicity
dc.subject
Lipid-Protein Binding
dc.subject.classification
Nano-materiales
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dc.subject.classification
Nanotecnología
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dc.subject.classification
INGENIERÍAS Y TECNOLOGÍAS
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dc.title
Ultraviolet irradiation of diacetylenic liposomes as a strategy to improve size stability and to alter protein binding without cytotoxicity enhancement
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2017-03-01T17:49:41Z
dc.identifier.eissn
1532-2394
dc.journal.volume
21
dc.journal.number
2
dc.journal.pagination
141-150
dc.journal.pais
Reino Unido
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dc.journal.ciudad
Londres
dc.description.fil
Fil: Temprana, Carlos Facundo. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnologia. Laboratorio de Biomembranas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
dc.description.fil
Fil: Amor, M. Silvia. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnologia. Laboratorio de Biomembranas; Argentina
dc.description.fil
Fil: Femia, Lis. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnologia. Laboratorio de Biomembranas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
dc.description.fil
Fil: Gasparri, Julieta. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnologia. Laboratorio de Biomembranas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
dc.description.fil
Fil: Taira, M. Cristina. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnologia. Laboratorio de Biomembranas; Argentina
dc.description.fil
Fil: Alonso, Silvia del Valle. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnologia. Laboratorio de Biomembranas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
dc.journal.title
Journal Of Liposome Research
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dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/http://www.tandfonline.com/doi/abs/10.3109/08982104.2010.492477?journalCode=ilpr20
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.3109/08982104.2010.492477
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