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dc.contributor.author
Yang, Sung Min  
dc.contributor.author
Alvarez, Diego  
dc.contributor.author
Schinder, Alejandro Fabián  
dc.date.available
2021-08-05T14:49:28Z  
dc.date.issued
2015-11  
dc.identifier.citation
Yang, Sung Min; Alvarez, Diego; Schinder, Alejandro Fabián; Reliable Genetic Labeling of Adult-Born Dentate Granule Cells Using Ascl1CreERT2 and GlastCreERT2 Murine Lines; Society for Neuroscience; Journal of Neuroscience; 35; 46; 11-2015; 15379-15390  
dc.identifier.issn
0270-6474  
dc.identifier.uri
http://hdl.handle.net/11336/137865  
dc.description.abstract
Newly generated dentate granule cells (GCs) are relevant for input discrimination in the adult hippocampus. Yet, their precise contribution to information processing remains unclear. To address this question, it is essential to develop approaches to precisely label entire cohorts of adult-born GCs. In this work, we used genetically modified mice to allow conditional expression of tdTomato (Tom) in adult-born GCs and characterized their development and functional integration. Ascl1CreERT2;CAGfloxStopTom and GlastCreERT2;CAGfloxStopTom mice resulted in indelible expression of Tom in adult neural stem cells and their lineage upon tamoxifen induction. Whole-cell recordings were performed to measure intrinsic excitability, firing behavior, and afferent excitatory connectivity. Developing GCs were also staged by the expression of early and late neuronal markers. The slow development of adult-born GCs characterized here is consistent with previous reports using retroviral approaches that have revealed that a mature phenotype is typically achieved after 6–8 weeks. Our findings demonstrate that Ascl1CreERT2 and GlastCreERT2 mouselines enable simpleandreliable labeling of adult-bornGClineages within restricted time windows. Therefore, these mice greatly facilitate tagging new neurons and manipulating their activity, required for understanding adult neurogenesis in the context of network remodeling, learning, and behavior.  
dc.format
application/pdf  
dc.language.iso
eng  
dc.publisher
Society for Neuroscience  
dc.rights
info:eu-repo/semantics/openAccess  
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/  
dc.subject
ADULT NEUROGENESIS  
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ELECTROPHYSIOLOGY  
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HIPPOCAMPUS  
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NEURAL CIRCUITS  
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SYNAPTOGENESIS  
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TRANSGENIC MICE  
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Otras Ciencias Médicas  
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Otras Ciencias Médicas  
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CIENCIAS MÉDICAS Y DE LA SALUD  
dc.title
Reliable Genetic Labeling of Adult-Born Dentate Granule Cells Using Ascl1CreERT2 and GlastCreERT2 Murine Lines  
dc.type
info:eu-repo/semantics/article  
dc.type
info:ar-repo/semantics/artículo  
dc.type
info:eu-repo/semantics/publishedVersion  
dc.date.updated
2021-07-01T17:34:31Z  
dc.identifier.eissn
1529-2401  
dc.journal.volume
35  
dc.journal.number
46  
dc.journal.pagination
15379-15390  
dc.journal.pais
Estados Unidos  
dc.journal.ciudad
Washington  
dc.description.fil
Fil: Yang, Sung Min. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquímicas de Buenos Aires. Fundación Instituto Leloir. Instituto de Investigaciones Bioquímicas de Buenos Aires; Argentina  
dc.description.fil
Fil: Alvarez, Diego. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquímicas de Buenos Aires. Fundación Instituto Leloir. Instituto de Investigaciones Bioquímicas de Buenos Aires; Argentina  
dc.description.fil
Fil: Schinder, Alejandro Fabián. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquímicas de Buenos Aires. Fundación Instituto Leloir. Instituto de Investigaciones Bioquímicas de Buenos Aires; Argentina  
dc.journal.title
Journal of Neuroscience  
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://www.jneurosci.org/content/35/46/15379.long  
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1523/JNEUROSCI.2345-15.2015