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Artículo

Cardiac up-regulation of NBCe1 emerges as a beneficial consequence of voluntary wheel running in mice

Medina, Andrés JavierIcon ; Ibañez, Alejandro MartinIcon ; Diaz Zegarra, Leandro AgustinIcon ; Portiansky, Enrique LeoIcon ; Blanco, Paula GracielaIcon ; Pereyra, Erica VanesaIcon ; de Giusti, Verónica CelesteIcon ; Aiello, Ernesto AlejandroIcon ; Yeves, Alejandra del MilagroIcon ; Ennis, Irene LuciaIcon
Fecha de publicación: 15/11/2020
Editorial: Elsevier Science Inc.
Revista: Archives of Biochemistry and Biophysics
ISSN: 0003-9861
e-ISSN: 1096-0384
Idioma: Inglés
Tipo de recurso: Artículo publicado
Clasificación temática:
Fisiología

Resumen

Physical training stimulates the development of physiologic cardiac hypertrophy (CH), being a key event in this process the inhibition of the Na+/H+ exchanger. However, the role of the sodium bicarbonate cotransporter (NBC) has not been explored yet under this circumstance. C57/Bl6 mice were allowed to voluntary exercise (wheel running) for five weeks. Cardiac mass was evaluated by echocardiography and histomorphometry detecting that training promoted the development of physiological CH (heart weight/tibia length ratio, mg/mm: 6.54 ± 0.20 vs 8.81 ± 0.24; interstitial collagen content, %: 3.14 ± 0.63 vs. 1.57 ± 0.27; and cross-sectional area of cardiomyocytes, μm2: 200.6 ± 8.92 vs. 281.9 ± 24.05; sedentary (Sed) and exercised (Ex) mice, respectively). The activity of the electrogenic isoform of the cardiac NBC (NBCe1) was estimated by recording intracellular pH under high potassium concentration and by measuring action potential duration (APD). NBCe1 activity was significantly increased in isolated cardiomyocytes of trained mice. Additionally, the APD was shorter and the alkalization due to high extracellular potassium-induced depolarization was greater in this group, indicating that the NBCe1 was hyperactive. These results are online with the observed myocardial up-regulation of the NBCe1 (Western Blot, %: 100 ± 13.86 vs. 202 ± 29.98; Sed vs. Ex, n = 6 each group). In addition, we detected a reduction in H2O2 production in the myocardium of trained mice. These results support that voluntary training induces the development of physiologic CH with up-regulation of the cardiac NBCe1 in mice. Furthermore, the improvement in the antioxidant capacity contributes to the beneficial cardiovascular consequences of physical training.
Palabras clave: EXERCISE , NBCE1 , OXIDATIVE STRESS , PHYSIOLOGIC CARDIAC HYPERTROPHY , VOLUNTARY WHEEL RUNNING
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info:eu-repo/semantics/restrictedAccess Excepto donde se diga explícitamente, este item se publica bajo la siguiente descripción: Creative Commons Attribution-NonCommercial-ShareAlike 2.5 Unported (CC BY-NC-SA 2.5)
Identificadores
URI: http://hdl.handle.net/11336/137686
URL: https://www.sciencedirect.com/science/article/abs/pii/S0003986120306093
DOI: https://doi.org/10.1016/j.abb.2020.108600
Colecciones
Articulos(CCT - LA PLATA)
Articulos de CTRO.CIENTIFICO TECNOL.CONICET - LA PLATA
Articulos(CIC)
Articulos de CENTRO DE INVEST.CARDIOVASCULARES (I)
Citación
Medina, Andrés Javier; Ibañez, Alejandro Martin; Diaz Zegarra, Leandro Agustin; Portiansky, Enrique Leo; Blanco, Paula Graciela; et al.; Cardiac up-regulation of NBCe1 emerges as a beneficial consequence of voluntary wheel running in mice; Elsevier Science Inc.; Archives of Biochemistry and Biophysics; 694; 15-11-2020; 1-11; 108600
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