Artículo
The neurotoxic effect of cuprizone on oligodendrocytes depends on the presence of pro-inflammatory cytokines secreted by microglia
Pasquini, Laura Andrea
; Calatayud, Cecilia Alicia
; Bertone Uña, A.L.; Millet, Violeta
; Pasquini, Juana Maria
; Soto, Eduardo Francisco
Fecha de publicación:
12/2007
Editorial:
Springer/Plenum Publishers
Revista:
Neurochemical Research
ISSN:
0364-3190
Idioma:
Inglés
Tipo de recurso:
Artículo publicado
Clasificación temática:
Resumen
In order to further characterize the still unknown mechanism of cuprizone-induced demyelination, we investigated its effect on rat primary oligodendroglial cell cultures. Cell viability was not significantly affected by this treatment. However, when concentrations of IFNγ and/or TNFα having no deleterious effects per se on cell viability were added together with cuprizone, cell viability decreased significantly. In mitochondria isolated from cuprizone-treated glial cells, we observed a marked decrease in the activities of the various complexes of the respiratory chain, indicating a disruption of mitochondrial function. An enhancement in oxidant production was also observed in cuprizone and/or TNFα-treated oligodendroglial cells. In in vivo experiments, inhibition of microglial activation with minocycline prevented cuprizone-induced demyelination. Based on the above-mentioned results we suggest that these microglial cells appear to have a very active role in cuprizone-induced oligodendroglial cell death and demyelination, through the production and secretion of pro-inflammatory cytokines.
Palabras clave:
CUPRIZONE
,
CYTOKINES
,
DEMYELINATION
,
MICROGLIA
,
MYELIN
,
OLIGODENDROCYTES
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Articulos(IQUIFIB)
Articulos de INST.DE QUIMICA Y FISICO-QUIMICA BIOLOGICAS "PROF. ALEJANDRO C. PALADINI"
Articulos de INST.DE QUIMICA Y FISICO-QUIMICA BIOLOGICAS "PROF. ALEJANDRO C. PALADINI"
Citación
Pasquini, Laura Andrea; Calatayud, Cecilia Alicia; Bertone Uña, A.L.; Millet, Violeta; Pasquini, Juana Maria; et al.; The neurotoxic effect of cuprizone on oligodendrocytes depends on the presence of pro-inflammatory cytokines secreted by microglia; Springer/Plenum Publishers; Neurochemical Research; 32; 2; 12-2007; 279-292
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