Artículo
New and rapid strategies for the diagnosis of bovine paratuberculosis “in situ” using latex particles
Alonso, Maria Natalia
; Garcia, Valeria Soledad
; Moyano, Roberto Damian
; Romero, Magali Andrea
; Gugliotta, Luis Marcelino
; Travería, Gabriel Eduardo; Romano, Maria Isabel
; González, Verónica Doris Guadalupe
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Fecha de publicación:
06/2021
Editorial:
Elsevier Science
Revista:
Journal Of Immunological Methods
ISSN:
0022-1759
Idioma:
Inglés
Tipo de recurso:
Artículo publicado
Clasificación temática:
Resumen
The chemical coupling of a protoplasmatic antigen from Mycobacterium avium subsp. paratubeculosis onto core-shell carboxylated particles was investigated with the aim of producing latex-protein complexes to be used in immunoagglutination assays capable of detecting bovine paratuberculosis disease. For this purpose, sensitizations were carried out using both colored and not colored carboxylated latexes as well as the protoplasmatic antigen at pH close to its isoelectric point to favor the antigenic protein to approach the particle surface. In all cases, higher fractions of proteins were chemically-bound to carboxyl groups on the surface of the particles. The assessment of the performance of the visual immunoagglutination assays consisted of evaluating 111 sera from healthy and infected bovines with Mycobacterium avium subsp. paratuberculosis. Complexes obtained from the colored latex allowed an acceptable visual discrimination between the studied positive and negative sera. Most of the positive samples showed strong to very strong agglutination and only a few samples reacted weakly, i.e. a sensitivity of 70%. The specificity of the assay, on the other hand, was 86%. Therefore, this rapid detection technique allows an easy and inexpensive identification of animals possibly infected with paratuberculosis “in situ” in the herds.
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Articulos (IABIMO)
Articulos de INSTITUTO DE AGROBIOTECNOLOGIA Y BIOLOGIA MOLECULAR
Articulos de INSTITUTO DE AGROBIOTECNOLOGIA Y BIOLOGIA MOLECULAR
Articulos(INTEC)
Articulos de INST.DE DES.TECNOL.PARA LA IND.QUIMICA (I)
Articulos de INST.DE DES.TECNOL.PARA LA IND.QUIMICA (I)
Citación
Alonso, Maria Natalia; Garcia, Valeria Soledad; Moyano, Roberto Damian; Romero, Magali Andrea; Gugliotta, Luis Marcelino; et al.; New and rapid strategies for the diagnosis of bovine paratuberculosis “in situ” using latex particles; Elsevier Science; Journal Of Immunological Methods; 496; 6-2021; 1-10
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