Artículo
Detection of bovine IgG isotypes in a PPA-ELISA for Johne's disease diagnosis in infected herds
Fernández, Bárbara
; Gilardoni, Liliana Rosa; Jolly, Ana
; Colavecchia, Silvia Beatriz; Paolicchi, Fernando Alberto; Mundo, Silvia Leonor
Fecha de publicación:
26/06/2012
Editorial:
Hindawi Limited
Revista:
Veterinary Medicine International
ISSN:
2042-0048
e-ISSN:
2090-8113
Idioma:
Inglés
Tipo de recurso:
Artículo publicado
Clasificación temática:
Resumen
Johne’s Disease or Paratuberculosis is a chronic granulomatous enteritis disease affecting ruminants. Detection of subclinically infected animals is difficult, hampering the control of this disease. The aim of this work was to evaluate the performance of detection of IgG isotypes in a PPA-ELISA to improve the recognition of cattle naturally infected with Map in different stages. A total of 108 animals from Tuberculosis-free herds were grouped as follows: exposed (n = 30), subclinically infected (n = 26), clinically infected (n = 14), and healthy controls (n = 38). Receiver-operating characteristic (ROC) curves of isotypes/PPA-ELISAs were constructed and areas under the curves were compared to evaluate the performance of each test. Our study demonstrated that the conventional PPA-ELISA (detecting IgG) is the best to identify clinically infected animals with high sensitivity (92.9%) and specificity (100%). Meanwhile, IgG2/PPA-ELISA improved the number of subclinically infected cattle detected as compared with conventional IgG/PPA-ELISA (53.8 versus 23.1%). In addition, it had the maximum sensitivity (65.0%, taking into account all Map-infected cattle). In conclusion, the combination of IgG and IgG2/PPA-ELISAs may improve the identification of Mapinfected cattle in different stages of disease. The usefulness of IgG2 detection in serological tests for Johne’s Disease diagnosis should be further evaluated.
Palabras clave:
PARATUBERCULOSIS
,
BOVINE
,
ANTIBODIES
,
ISOTYPES
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Articulos(OCA PQUE. CENTENARIO)
Articulos de OFICINA DE COORDINACION ADMINISTRATIVA PQUE. CENTENARIO
Articulos de OFICINA DE COORDINACION ADMINISTRATIVA PQUE. CENTENARIO
Citación
Fernández, Bárbara; Gilardoni, Liliana Rosa; Jolly, Ana; Colavecchia, Silvia Beatriz; Paolicchi, Fernando Alberto; et al.; Detection of bovine IgG isotypes in a PPA-ELISA for Johne's disease diagnosis in infected herds; Hindawi Limited; Veterinary Medicine International; 2012; 26-6-2012; 1-6
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