Artículo
Gametogenesis and reproductive dynamics of scinax acuminatus (Anura: Hylidae): Morphological, histological and immunohistochemical analysis
Olea, Gabriela Beatriz
; Cheij, Esteban Omar; Cuzziol Boccioni, Ana Paula
; Rodriguez, Florencia Evelyn
; Céspedez, Jorge; Lombardo, Daniel Marcelo
Fecha de publicación:
2021
Editorial:
Academia Brasileira de Ciencias
Revista:
Anais da Academia Brasileira de Ciencias
ISSN:
0001-3765
e-ISSN:
1678-2690
Idioma:
Inglés
Tipo de recurso:
Artículo publicado
Clasificación temática:
Resumen
To characterize the reproductive biology of Scinax acuminatus and contribute to the natural history of this species, the morphology of the reproductive system of males and females was analyzed at anatomical, histological and immunohistochemical levels. The individuals were collected fortnightly between August and December (2016) and January to December (2018). The anatomy of the reproductive system was analyzed in a stereoscopic microscope, and histological preparations staining with hematoxylin-eosin and Masson’s trichromic, PAS and Coomassie Blue was performed as well. To characterize the meiotic-active cells in the testes, immunostaining with the PCNA proliferation protein was performed. There were found females with ovaries with oocytes in different stages of maturity and post-ovulatory females. Males presented continuous spermatogenesis, which could be confirmed by the immunostaining of PCNA in spermatogonia during the cycle. The results of this work serve as a basis for the characterization of the reproductive cycle in S. acuminatus and provide background information on the analysis of spermatogenic activity by IHQ from the study of the immunodetection of the PCNA cell proliferation protein. Future studies will focus on the evaluation of cell death processes during the reproductive cycle in the studied species to compare with those obtained in terms of cell proliferation.
Palabras clave:
AMPHIBIANS
,
OVARIES
,
REPRODUCTION
,
TESTICLES
Archivos asociados
Licencia
Identificadores
Colecciones
Articulos(OCA PQUE. CENTENARIO)
Articulos de OFICINA DE COORDINACION ADMINISTRATIVA PQUE. CENTENARIO
Articulos de OFICINA DE COORDINACION ADMINISTRATIVA PQUE. CENTENARIO
Citación
Olea, Gabriela Beatriz; Cheij, Esteban Omar; Cuzziol Boccioni, Ana Paula; Rodriguez, Florencia Evelyn; Céspedez, Jorge; et al.; Gametogenesis and reproductive dynamics of scinax acuminatus (Anura: Hylidae): Morphological, histological and immunohistochemical analysis; Academia Brasileira de Ciencias; Anais da Academia Brasileira de Ciencias; 93; 2; 2021; 1-11
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