Artículo
Developmental expression of genes involved in progesterone synthesis, metabolism and action during the post-natal cerebellar myelination
Mancino, Dalila NJ; Leicaj, Maria Luz
; Lima, Analia Ethel
; Roig, Paulina
; Guennoun, Rachida; Schumacher, Michael; de Nicola, Alejandro Federico
; Garay, Laura Ines
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Fecha de publicación:
03/2021
Editorial:
Elsevier Ltd.
Revista:
Journal of Steroid Biochemistry and Molecular Biology
ISSN:
0960-0760
Idioma:
Inglés
Tipo de recurso:
Artículo publicado
Clasificación temática:
Resumen
Progesterone is involved in dendritogenesis, synaptogenesis and maturation of cerebellar Purkinge cells, major sites of steroid synthesis in the brain. To study a possible time-relationship between myelination, neurosteroidogenesis and steroid receptors during development of the postnatal mouse cerebellum, we determined at postnatal days 5 (P5),18 (P18) and 35 (P35) the expression of myelin basic protein (MBP), components of the steroidogenic pathway, levels of endogenous steroids and progesteroneʾs classical and non-classical receptors. In parallel with myelin increased expression during development, P18 and P35 mice showed higher levels of cerebellar progesterone and its reduced derivatives, higher expression of steroidogenic acute regulatory protein (StAR) mRNA, cholesterol side chain cleavage enzyme (P450scc) and 5α-reductase mRNA vs. P5 mice. Other steroids such as corticosterone and its reduced derivatives and 3β-androstanodiol (ADIOL) showed a peak increase at P18 compared to P5. Progesterone membrane receptors and binding proteins (PGRMC1, mPRα, mPRβ, mPRγ, and Sigma1 receptors) mRNAs levels increased during development while that of classical progesterone receptors (PR) remained invariable. PRKO mice showed similar MBP levels than wild type. Thus, these data suggests that progesterone and its neuroactive metabolites may play a role in postnatal cerebellar myelination.
Palabras clave:
CEREBELLUM
,
MYELINATION
,
NEUROSTEROIDS
,
POSTNATAL DEVELOPMENT
,
STEROID RECEPTORS
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Identificadores
Colecciones
Articulos(IBYME)
Articulos de INST.DE BIOLOGIA Y MEDICINA EXPERIMENTAL (I)
Articulos de INST.DE BIOLOGIA Y MEDICINA EXPERIMENTAL (I)
Articulos(INBIRS)
Articulos de INSTITUTO DE INVESTIGACIONES BIOMEDICAS EN RETROVIRUS Y SIDA
Articulos de INSTITUTO DE INVESTIGACIONES BIOMEDICAS EN RETROVIRUS Y SIDA
Citación
Mancino, Dalila NJ; Leicaj, Maria Luz; Lima, Analia Ethel; Roig, Paulina; Guennoun, Rachida; et al.; Developmental expression of genes involved in progesterone synthesis, metabolism and action during the post-natal cerebellar myelination; Elsevier Ltd.; Journal of Steroid Biochemistry and Molecular Biology; 207; 3-2021; 105820-105820
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