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dc.contributor.author
Wigmann, Évelin F.
dc.contributor.author
Meyer, Karsten
dc.contributor.author
Cendoya, Eugenia
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dc.contributor.author
Maul, Ronald
dc.contributor.author
Vogel, Rudi F.
dc.contributor.author
Niessen, Ludwig
dc.date.available
2021-07-26T19:00:09Z
dc.date.issued
2020-07
dc.identifier.citation
Wigmann, Évelin F.; Meyer, Karsten; Cendoya, Eugenia; Maul, Ronald; Vogel, Rudi F.; et al.; A loop-mediated isothermal amplification (LAMP) based assay for the rapid and sensitive group-specific detection of fumonisin producing Fusarium spp; Elsevier Science; International Journal of Food Microbiology; 325; 7-2020; 1-12
dc.identifier.issn
0168-1605
dc.identifier.uri
http://hdl.handle.net/11336/136970
dc.description.abstract
Fumonisins are mycotoxins that contaminate maize and maize-based food products, and feed. They have been associated with nerve system disorders in horses, pulmonary edema in swine as well as neural tube defects and esophageal cancer in humans. The fum1 gene codes for a polyketide synthase involved in the biosynthesis of fumonisins. It is present in the genomes of all fumonisin producing Fusarium spp. Reliable detection of fum1 can provide an estimate of the toxicological potential of cultures and food sources. Therefore, a fum1 specific LAMP assay was developed and tested with purified DNA of 48 different species from the Fusarium fujikuroi species complex (FFSC). The fum1 gene was detected in 22 species among which F. fujikuroi, F. globosum, F. nygamai, F. proliferatum, F. subglutinans and F. verticillioides were the most prominent fumonisin producers. None out of 92 tested non-Fusarium species showed cross reactions with the new assay. The lowest limit of detection (LOD) was 5 pg of genomic DNA per reaction for F. fujikuroi, F. nygamai and F. verticillioides. Higher LODs were found for other LAMP positive species. Apart from pure genomic DNA, the LAMP assay detected fumonisin-producers when 103 conidia/reaction were used as template after mechanical lysis. LAMP-results were well correlated with FB1 production. This is the first report on fumonisin production in strains of F. annanatum, F. coicis, F. mundagurra, F. newnesense, F. pininemorale, F. sororula, F. tjataeba, F. udum and F. werrikimbe. Usefulness of the LAMP assay was demonstrated by analyzing fumonisin contaminated maize grains. The new LAMP assay is rapid, sensitive and reliable for the diagnosis of typical fumonisin producers and can be a versatile tool in HACCP concepts that target the reduction of fumonisins in the food and feed chain.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Elsevier Science
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dc.rights
info:eu-repo/semantics/restrictedAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
DIAGNOSIS
dc.subject
FFSC
dc.subject
FUM1
dc.subject
GENE DETECTION
dc.subject
MYCOTOXIN
dc.subject.classification
Micología
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dc.subject.classification
Ciencias Biológicas
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dc.subject.classification
CIENCIAS NATURALES Y EXACTAS
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dc.title
A loop-mediated isothermal amplification (LAMP) based assay for the rapid and sensitive group-specific detection of fumonisin producing Fusarium spp
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2021-06-07T15:36:14Z
dc.journal.volume
325
dc.journal.pagination
1-12
dc.journal.pais
Países Bajos
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dc.journal.ciudad
Amsterdam
dc.description.fil
Fil: Wigmann, Évelin F.. Universitat Technical Zu Munich; Alemania
dc.description.fil
Fil: Meyer, Karsten. Technische Universitat München; Alemania
dc.description.fil
Fil: Cendoya, Eugenia. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Instituto de Investigación en Micología y Micotoxicología. - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigación en Micología y Micotoxicología; Argentina
dc.description.fil
Fil: Maul, Ronald. German Federal Institute For Risk Assessment; Alemania
dc.description.fil
Fil: Vogel, Rudi F.. Universitat Technical Zu Munich; Alemania
dc.description.fil
Fil: Niessen, Ludwig. Universitat Technical Zu Munich; Alemania
dc.journal.title
International Journal of Food Microbiology
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dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://linkinghub.elsevier.com/retrieve/pii/S0168160520301215
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1016/j.ijfoodmicro.2020.108627
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