Artículo
Treatment with melatonin after onset of experimental uveitis attenuates ocular inflammation
Sande, Pablo Horacio; Dorfman, Damián
; Fernandez, Diego Carlos
; Chianelli, Monica Silvia
; Domínguez Rubio, Ana Paula
; Franchi, Ana Maria
; Silberman, Dafne Magali
; Rosenstein, Ruth Estela
; Saenz, Daniel Alberto
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Fecha de publicación:
12/2014
Editorial:
Wiley
Revista:
British Journal of Pharmacology
ISSN:
0007-1188
Idioma:
Inglés
Tipo de recurso:
Artículo publicado
Clasificación temática:
Resumen
Background and Purpose: Uveitis is a prevalent intraocular inflammatory disease and one of the most damaging ocular conditions. Pretreatment with melatonin prevented ocular inflammation induced by an intravitreal injection of bacterial LPS in the Syrian hamster. Here, we have assessed the anti-inflammatory effects of melatonin administered after the onset of ocular inflammation. Experimental Approach: The eyes of male Syrian hamsters were intravitreally injected with vehicle or LPS. Melatonin was injected i.p. every 24 h, starting 12 or 24 h after the LPS injection. A clinical evaluation (with a score index based on clinical symptoms), the number of infiltrating cells, protein concentration and PGE2 and PGF2α levels in the aqueous humour, as well as retinal NOS activity, lipid peroxidation and TNF-α levels were assessed. Retinal function was assessed by scotopic electroretinography, and light microscopy and immunohistochemistry were used to evaluate the state of the retinal structure. Key Results: Both treatment regimens with melatonin decreased clinical symptoms, reduced the leakage of cells and proteins, and decreased PG levels in aqueous humour from eyes injected with LPS. In addition, melatonin treatment blocked the decrease in scotopic electroretinogram a- and b-wave amplitude, protected the retinal structure and reduced the increase in NOS activity, lipid peroxidation and TNF-α levels, induced by LPS. Conclusions and Implications: These results indicate that treatment with melatonin, starting after the onset of uveitis, attenuated ocular inflammation induced by LPS in the Syrian hamster and support the use of melatonin as a therapeutic resource for uveitis treatment.
Palabras clave:
Melatonin
,
Ocular Inflammation
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Identificadores
Colecciones
Articulos(CEFYBO)
Articulos de CENTRO DE ESTUDIOS FARMACOLOGICOS Y BOTANICOS
Articulos de CENTRO DE ESTUDIOS FARMACOLOGICOS Y BOTANICOS
Citación
Sande, Pablo Horacio; Dorfman, Damián; Fernandez, Diego Carlos; Chianelli, Monica Silvia; Domínguez Rubio, Ana Paula; et al.; Treatment with melatonin after onset of experimental uveitis attenuates ocular inflammation; Wiley; British Journal of Pharmacology; 171; 24; 12-2014; 5696-5707
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