Artículo
Photodynamic therapy of glioblastoma cells using doped conjugated polymer nanoparticles: An in vitro comparative study based on redox status
Caverzan, Matias Daniel
; Beaugé, Lucía
; Chesta, Carlos Alberto
; Palacios, Rodrigo Emiliano
; Ibarra, Luis Exequiel
Fecha de publicación:
11/2020
Editorial:
Elsevier Science SA
Revista:
Journal of Photochemistry and Photobiology B: Biology
ISSN:
1011-1344
Idioma:
Inglés
Tipo de recurso:
Artículo publicado
Clasificación temática:
Resumen
Due to their superb light absorption and photostability conjugated polymer nanoparticles are promising photosensitizers (PS) for their use in Photodynamic therapy (PDT). Recently, we developed metallated porphyrin-doped conjugated polymer nanoparticles (CPNs) for PDT that efficiently eliminate tumor cells through reactive oxygen species (ROS) mediated photoinduced damage of apoptotic nature. These nanoaggregates act as densely packed multi-chromophoric systems having exceptional light harvesting and (intra-particle) energy transfer capabilities which lead to efficient photosensitized formation of ROS. In general, three key components; light, PS, and oxygen; are considered in the prediction of the PDT outcome. However, recent studies led to the discovery of a profound genetic heterogeneity among glioblastoma (GBM) cells which include the adaptation to ROS. Thus, tumor heterogeneity and their associated difference in sensitivity to ROS-producing therapeutic agents must be considered in the design of PDT protocols for the prediction of its outcome. In this study, anticancer activity through ROS-mediated PDT using CPNs was compared in three GBM cell lines with different initial redox status. T98G cells were the most effective incorporating nanoparticles but also were the most resistant to CPN-PDT effect. In part, this feature could be attributed to the differential basal and PDT-induced antioxidant enzyme levels found in these cells measured by gene expression analysis. Furthermore, considering that cell-specific antioxidant enzyme status is a significant feature of GBM heterogeneity, establishing its correlation with CPN-PDT outcome might be important for designing novel and improved CPN-based treatments.
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Colecciones
Articulos (IITEMA)
Articulos de INSTITUTO DE INVESTIGACIONES EN TECNOLOGIAS ENERGETICAS Y MATERIALES AVANZADOS
Articulos de INSTITUTO DE INVESTIGACIONES EN TECNOLOGIAS ENERGETICAS Y MATERIALES AVANZADOS
Articulos (INBIAS)
Articulos de INSTITUTO DE BIOTECNOLOGIA AMBIENTAL Y SALUD
Articulos de INSTITUTO DE BIOTECNOLOGIA AMBIENTAL Y SALUD
Citación
Caverzan, Matias Daniel; Beaugé, Lucía; Chesta, Carlos Alberto; Palacios, Rodrigo Emiliano; Ibarra, Luis Exequiel; Photodynamic therapy of glioblastoma cells using doped conjugated polymer nanoparticles: An in vitro comparative study based on redox status; Elsevier Science SA; Journal of Photochemistry and Photobiology B: Biology; 212; 11-2020; 1-9
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