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dc.contributor.author
Di Rosso, María Emilia
dc.contributor.author
Barreiro Arcos, María Laura
dc.contributor.author
Elingold, Igal
dc.contributor.author
Sterle, Helena Andrea
dc.contributor.author
Ferreira, Sabrina Baptista
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Ferreira, Vitor Francisco
dc.contributor.author
Galleano, Monica Liliana
dc.contributor.author
Cremaschi, Graciela Alicia
dc.contributor.author
Dubin, Marta
dc.date.available
2017-03-08T21:45:53Z
dc.date.issued
2013-10
dc.identifier.citation
Di Rosso, María Emilia; Barreiro Arcos, María Laura; Elingold, Igal; Sterle, Helena Andrea; Ferreira, Sabrina Baptista; et al.; Novel o-naphthoquinones induce apoptosis of EL-4 T lymphoma cells through the increase of reactive oxygen species; Elsevier; Toxicology In Vitro; 27; 7; 10-2013; 2094-2104
dc.identifier.issn
0887-2333
dc.identifier.uri
http://hdl.handle.net/11336/13661
dc.description.abstract
Novel β-lapachone analogs 2-phenyl-3,4-dihydro-2H-benzo[h]chromene-5,6-dione (NQ1), 2-p-tolyl-3,4-dihydro-2H-benzo[h]chromene-5,6-dione (NQ3) and 2-methyl-2-phenyl-3,4-dihydro-2H-benzo[h]chromene-5,6-dione (NQ7), which have trypanocidal activity, were assayed for cytotoxic effects on murine EL-4 T lymphoma cells. The NQs inhibited the proliferation of EL-4 cells at concentrations above 1 μM. Nuclear staining of the EL-4 cells revealed chromatin condensation and a nuclear morphology compatible with the induction of apoptosis. Flow cytometry assays with annexin V-FITC and propidium iodide confirmed the cell death by apoptosis. Using electron paramagnetic resonance (EPR), a semiquinone radical was detected in EL-4 cells treated with NQs. In addition, a decrease in the GSH level in parallel with reactive oxygen species (ROS) production was observed. Preincubation with n-acetyl-l-cysteine (NAC) was able to reverse the inhibitory effects of the NQs on cell proliferation, indicating that ROS generation is involved in NQ-induced apoptosis. In addition, the NQs induced a decrease in the mitochondrial membrane potential and increased the proteolytic activation of caspases 9 and 3 and the cleavage of Poly (ADP-Ribose) Polymerase (PARP). In conclusion, these results indicate that redox cycling is induced by the NQs in the EL-4 cell line, with the generation of ROS and other free radicals that could inhibit cellular proliferation as a result of the induction of the intrinsic apoptosis pathway.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Elsevier
dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
dc.subject
O-Naphthoquinones
dc.subject
Oxidative Stress
dc.subject
Apoptosis
dc.subject.classification
Biología Celular, Microbiología
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Ciencias Biológicas
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CIENCIAS NATURALES Y EXACTAS
dc.title
Novel o-naphthoquinones induce apoptosis of EL-4 T lymphoma cells through the increase of reactive oxygen species
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2017-03-06T18:06:10Z
dc.journal.volume
27
dc.journal.number
7
dc.journal.pagination
2094-2104
dc.journal.pais
Países Bajos
dc.journal.ciudad
Ámsterdam
dc.description.fil
Fil: Di Rosso, María Emilia. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Centro de Estudios Farmacológicos y Botánicos; Argentina. Universidad de Buenos Aires. Facultad de Medicina; Argentina
dc.description.fil
Fil: Barreiro Arcos, María Laura. Universidad Catolica Argentina; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
dc.description.fil
Fil: Elingold, Igal. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Centro de Estudios Farmacológicos y Botánicos; Argentina. Universidad de Buenos Aires. Facultad de Medicina; Argentina
dc.description.fil
Fil: Sterle, Helena Andrea. Pontificia Universidad Católica Argentina "Santa María de Los Buenos Aires"; Argentina
dc.description.fil
Fil: Ferreira, Sabrina Baptista. Universidade Federal Do Rio de Janeiro; Brasil
dc.description.fil
Fil: Ferreira, Vitor Francisco. Universidade Federal Fluminense; Brasil
dc.description.fil
Fil: Galleano, Monica Liliana. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Bioquímica y Medicina Molecular; Argentina
dc.description.fil
Fil: Cremaschi, Graciela Alicia. Pontificia Universidad Católica Argentina "Santa María de Los Buenos Aires"; Argentina
dc.description.fil
Fil: Dubin, Marta. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Centro de Estudios Farmacológicos y Botánicos; Argentina. Universidad de Buenos Aires. Facultad de Medicina; Argentina
dc.journal.title
Toxicology In Vitro
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0887233313001914
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1016/j.tiv.2013.08.002
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