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Artículo

Cryopreservation of a Human Brain and Its Experimental Correlate in Rats

Canatelli Mallat, MartinaIcon ; Lascaray, Francisco; Entraigues Abramson, Maria; Portiansky, Enrique LeoIcon ; Balmaceda, Néstor; Morel, Gustavo RamónIcon ; Goya, Rodolfo GustavoIcon
Fecha de publicación: 12/2020
Editorial: Mary Ann Liebert
Revista: Rejuvenation Research
ISSN: 1549-1684
Idioma: Inglés
Tipo de recurso: Artículo publicado
Clasificación temática:
Otras Ciencias de la Salud

Resumen

Several countries have established self-help cryonics groups whose mission is to cryopreserve human bodies or brains after legal death and ship them to cryonics organizations. The objective of this study was to report the first case of human brain cryopreservation in Argentina and complementary experiments in rats. After legal death, the body of a 78-year-old Caucasian woman was transported to a funeral home where her head was submitted to intracarotid perfusion with 5 L cold physiologic saline followed by the same volume of cold saline containing 13% dimethyl sulfoxide and 13% glycerol. The brain was removed, temporarily frozen at-80°C, and shipped to a U.S. cryostasis facility. Three groups of rats were intracardially perfused with fixative but not frozen (Reference group), vitrification solution VM1 (Control group), or the cryoprotection solution used in the patient (Experimental group). Control and Experimental brains were stored at-80°C and subsequently assessed by immunohistochemistry for the adult neuron marker (NeuN), the immature neuron marker doublecortin (DCX), the dopaminergic neuron marker tyrosine hydroxylase, and the presynaptic marker synaptophysin (SYN). The number of NeuN-positive neurons remained unchanged in the experimental brain cortex, whereas the number of immature DCX neurons in the hippocampus fell markedly in the cryoprotected brains. The results were highly variable for hypothalamic dopaminergic neurons. Confocal microscopy for SYN revealed that cryopreservation did not affect the synaptic network in the hippocampus. To our knowledge, this is the first report correlating a human cryoprotection procedure with results in complementary experiments in laboratory animals.
Palabras clave: BRAIN , CRYOPROTECTION , HUMAN , RAT , SYNAPTIC NETWORK , VITRIFICATION
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info:eu-repo/semantics/restrictedAccess Excepto donde se diga explícitamente, este item se publica bajo la siguiente descripción: Creative Commons Attribution-NonCommercial-ShareAlike 2.5 Unported (CC BY-NC-SA 2.5)
Identificadores
URI: http://hdl.handle.net/11336/136603
URL: https://www.liebertpub.com/doi/10.1089/rej.2019.2245
DOI: http://dx.doi.org/10.1089/rej.2019.2245
Colecciones
Articulos(CCT - LA PLATA)
Articulos de CTRO.CIENTIFICO TECNOL.CONICET - LA PLATA
Articulos(INIBIOLP)
Articulos de INST.DE INVEST.BIOQUIMICAS DE LA PLATA
Citación
Canatelli Mallat, Martina; Lascaray, Francisco; Entraigues Abramson, Maria; Portiansky, Enrique Leo; Balmaceda, Néstor; et al.; Cryopreservation of a Human Brain and Its Experimental Correlate in Rats; Mary Ann Liebert; Rejuvenation Research; 23; 6; 12-2020; 516-525
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