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dc.contributor.author
Malbrán, Ismael
dc.contributor.author
Mourelos, Cecilia Alejandra
dc.contributor.author
Lori, Gladys
dc.date.available
2021-07-21T17:52:44Z
dc.date.issued
2020-03
dc.identifier.citation
Malbrán, Ismael; Mourelos, Cecilia Alejandra; Lori, Gladys; First report of Fusarium oxysporum f. sp. lycopersici Race 3 Causing Fusarium Wilt of Tomato in Argentina; American Phytopathological Society; Plant Disease; 104; 3; 3-2020; 978-978
dc.identifier.issn
0191-2917
dc.identifier.uri
http://hdl.handle.net/11336/136594
dc.description.abstract
More than 15,000 ha of tomato (Solanum lycopersicum L.) are annually cultivated under greenhouse and field conditions in Argentina. From December 2013 to December 2016, plants of cultivar ‘Elpida’ showing wilting and stunting were observed in commercial fields from Goya, Corrientes. This province concentrates the largest area of greenhouses dedicated to tomato production in the country. Symptoms began as a yellowing of the lower leaves, which frequently was confined to one side of the plant at first and then spread to the rest of it. When stems of diseased plants were cut lengthwise, necrosis of the vascular tissue was observed, whereas the medulla remained healthy. Isolations were made from surface-sterilized sections of the vascular tissue, and a total of 14 monosporic isolates were obtained and identified as Fusarium oxysporum Schltdl. based on morphology and species-specific PCR (Hirano and Arie 2006). To identify the formae speciales and races of these isolates, pathogenicity tests were conducted on differential cultivars: ‘IPA-5’ (resistant to race 1), ‘Floradade’ and Elpida (resistant to races 1 and 2), ‘BHRS-2,3’ (resistant to races 1, 2 and 3), and ‘Ponderosa’ (susceptible to all races) (Reis et al. 2005). Isolates were incubated at 25 ± 2°C in 12 h light and 12 h dark on potato dextrose agar medium for 10 days. The obtained conidia were washed with autoclaved distilled water, and their concentration was adjusted to ≈106 microconidia/ml. For every isolate, four healthy seedlings of each cultivar showing the first two pairs of true leaves fully opened were removed from 35-cell germination trays, and the substrate was gently washed. The apical sector of the root was cut up to a length of 2 cm, and the remains were dipped in the spore suspension of one of the isolates for 1 min. To prepare the controls, roots were cut from four plants of each cultivar and dipped in sterilized distilled water. Afterward, the seedlings were transferred to 0.5-liter pots containing a mixture of tindalized soil, compost, and perlite (5:4:1 v/v) and grown in a greenhouse under natural daylight. Plants were evaluated 28 days postinoculation. All isolates induced typical symptoms of Fusarium wilt of tomato on cultivars Ponderosa, IPA-5, Elpida, and Floradade but not on cultivar BHRS-2,3 and hence were identified as F. oxysporum f. sp. lycopersici (Sacc.) W.C. Snyder & H.N. Hansen race 3. The pathogen was reisolated from vascular tissues of symptomatic plants from the three susceptible cultivars but not from control plants. To our knowledge, this is the first formal report of F. oxysporum f. sp. lycopersici race 3 in Corrientes, Argentina. The pathogen constitutes a potential threat to tomato production in this important area of production, and the knowledge of its presence might aid farmers in the selection of cultivars.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
American Phytopathological Society
dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
FUNGI
dc.subject
EPIDEMIOLOGY
dc.subject
FIELD CROPS
dc.subject.classification
Agronomía, reproducción y protección de plantas
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Agricultura, Silvicultura y Pesca
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CIENCIAS AGRÍCOLAS
dc.title
First report of Fusarium oxysporum f. sp. lycopersici Race 3 Causing Fusarium Wilt of Tomato in Argentina
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2021-07-15T12:23:56Z
dc.identifier.eissn
1943-7692
dc.journal.volume
104
dc.journal.number
3
dc.journal.pagination
978-978
dc.journal.pais
Estados Unidos
dc.description.fil
Fil: Malbrán, Ismael. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Agrarias y Forestales. Departamento de Ciencias Biológicas. Centro de Investigaciones de Fitopatología. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas. Centro de Investigaciones de Fitopatología; Argentina
dc.description.fil
Fil: Mourelos, Cecilia Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Agrarias y Forestales. Departamento de Ciencias Biológicas. Centro de Investigaciones de Fitopatología. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas. Centro de Investigaciones de Fitopatología; Argentina
dc.description.fil
Fil: Lori, Gladys. Universidad Nacional de La Plata. Facultad de Ciencias Agrarias y Forestales. Departamento de Ciencias Biológicas. Centro de Investigaciones de Fitopatología. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas. Centro de Investigaciones de Fitopatología; Argentina
dc.journal.title
Plant Disease
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://apsjournals.apsnet.org/doi/full/10.1094/PDIS-08-19-1777-PDN
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1094/PDIS-08-19-1777-PDN
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