Mostrar el registro sencillo del ítem
dc.contributor.author
Tizzano, Marco Antonio
dc.contributor.author
Sguazza, Guillermo Hernán
dc.contributor.author
Picotto, Leandro Daniel
dc.contributor.author
Echeverria, Maria Gabriela
dc.contributor.author
Pecoraro, Marcelo Ricardo
dc.date.available
2021-07-21T17:40:44Z
dc.date.issued
2020-05
dc.identifier.citation
Tizzano, Marco Antonio; Sguazza, Guillermo Hernán; Picotto, Leandro Daniel; Echeverria, Maria Gabriela; Pecoraro, Marcelo Ricardo; The cloning of the virus envelope glycoprotein F of canine distemper virus expressed in Pichia pastoris; Academic Press Ltd - Elsevier Science Ltd; Microbial Pathogenesis; 142; 5-2020; 1-17; 104094
dc.identifier.issn
0882-4010
dc.identifier.uri
http://hdl.handle.net/11336/136593
dc.description.abstract
Canine distemper virus (CDV) is a pathogen which affects members of the Canidae family, causing an acute, often fatal, systemic disease. CDV is an RNA virus of the family Paramyxoviridae that contains two envelope glycoproteins: F and HA. In this study, we focused on the envelope glycoprotein F as the main target for neutralizing antibodies produced after infection or vaccination. The complete coding region of the protein (60 kDa) was expressed in the methylotrophic yeast Pichia pastoris, obtained in a recombinant form and secreted to the culture medium. Later, to analyze its immunogenicity, the protein was combined with an oily adjuvant and used to inoculate mice. The results provide evidence supporting a potential application of this recombinant protein as a subunit vaccine.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Academic Press Ltd - Elsevier Science Ltd
dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
dc.subject
CANINE DISTEMPER VIRUS
dc.subject
GLYCOPROTEIN F
dc.subject
PICHIA PASTORIS
dc.subject.classification
Otras Ciencias Veterinarias
dc.subject.classification
Ciencias Veterinarias
dc.subject.classification
CIENCIAS AGRÍCOLAS
dc.title
The cloning of the virus envelope glycoprotein F of canine distemper virus expressed in Pichia pastoris
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2021-07-15T12:26:52Z
dc.identifier.eissn
1096-1208
dc.journal.volume
142
dc.journal.pagination
1-17; 104094
dc.journal.pais
Estados Unidos
dc.description.fil
Fil: Tizzano, Marco Antonio. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias; Argentina
dc.description.fil
Fil: Sguazza, Guillermo Hernán. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias; Argentina
dc.description.fil
Fil: Picotto, Leandro Daniel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias; Argentina
dc.description.fil
Fil: Echeverria, Maria Gabriela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias; Argentina
dc.description.fil
Fil: Pecoraro, Marcelo Ricardo. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias; Argentina
dc.journal.title
Microbial Pathogenesis
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/abs/pii/S0882401019321278
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/https://doi.org/10.1016/j.micpath.2020.104094
Archivos asociados