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dc.contributor.author
Rival, Claudia
dc.contributor.author
Guazzone, Vanesa Anabella
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dc.contributor.author
von Wulffen, Werner
dc.contributor.author
Hackstein, Holger
dc.contributor.author
Schneider, Eva
dc.contributor.author
Lustig, Livia
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dc.contributor.author
Meinhardt A.
dc.contributor.author
Fijak, Monika
dc.date.available
2021-07-16T17:57:08Z
dc.date.issued
2007-12
dc.identifier.citation
Rival, Claudia; Guazzone, Vanesa Anabella; von Wulffen, Werner; Hackstein, Holger; Schneider, Eva; et al.; Expression of co-stimulatory molecules, chemokine receptors and proinflammatory cytokines in dendritic cells from normal and chronically inflamed rat testis; Oxford University Press; Molecular Human Reproduction; 13; 12; 12-2007; 853-861
dc.identifier.issn
1360-9947
dc.identifier.uri
http://hdl.handle.net/11336/136349
dc.description.abstract
The presentation of self antigens by dendritic cells (DC) plays an important role in the initiation and maintenance of autoimmunity. In a model of experimental autoimmune orchitis (EAO), we have previously characterized dominant testicular autoantigens and shown an increase in DC numbers during the course of disease. In this study, we have developed a protocol for the isolation of a highly pure population of DC (∼97%) from the testis of EAO and control rats to analyse the expression of major histocompatibility complex (MHC) class II and co-stimulatory molecules (CD80, CD86), chemokine receptors (CCR2, CCR7) and cytokines (IL-10, IL-12p70, TNF-α). By flow cytometry, we observed similar percentage and intensity levels of MHC class II, CD80 and CD86 expression in testicular DC in all groups. Moreover, by real-time RT-PCR we have detected significantly higher CCR7 mRNA level in isolated testicular DC from rats with EAO compared to controls, whereas the expression of CCR2 was decreased in orchitis. Transcripts of IL-12p40 were observed in DC from all groups, whereas the expression of IL-10 and the rate limiting IL-12 subunit p35 were detectable exclusively in testicular DC from the inflamed testes. In co-culture experiments, testicular DC isolated from EAO animals significantly enhanced naïve T-cell proliferation compared with control DC. Taken together these results suggest that testicular DC in control testis is not mature and functionally tolerogenic, whereas in EAO testis, IL-12 expression and stimulation of T-cell proliferation points to a mature immunogenic state prior imminent migration to the lymph nodes to amplify immune responses against testicular antigens.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Oxford University Press
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dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
CO-STIMULATORY MOLECULES
dc.subject
CYTOKINES
dc.subject
DENDRITIC CELLS
dc.subject
EXPERIMENTAL AUTOIMMUNE ORCHITIS (EAO)
dc.subject
TESTICULAR INFLAMMATION
dc.subject.classification
Otras Ciencias Médicas
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dc.subject.classification
Otras Ciencias Médicas
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dc.subject.classification
CIENCIAS MÉDICAS Y DE LA SALUD
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dc.title
Expression of co-stimulatory molecules, chemokine receptors and proinflammatory cytokines in dendritic cells from normal and chronically inflamed rat testis
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2020-09-03T16:59:13Z
dc.journal.volume
13
dc.journal.number
12
dc.journal.pagination
853-861
dc.journal.pais
Reino Unido
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dc.journal.ciudad
Oxford
dc.description.fil
Fil: Rival, Claudia. Universidad de Buenos Aires; Argentina
dc.description.fil
Fil: Guazzone, Vanesa Anabella. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Biomédicas. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones Biomédicas; Argentina
dc.description.fil
Fil: von Wulffen, Werner. University of Giessen Lung Center; Alemania
dc.description.fil
Fil: Hackstein, Holger. Institute for Clinical Immunology and Transfusion Medicine; Alemania
dc.description.fil
Fil: Schneider, Eva. Justus Liebig Universitat Giessen; Alemania
dc.description.fil
Fil: Lustig, Livia. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Biomédicas. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones Biomédicas; Argentina
dc.description.fil
Fil: Meinhardt A.. Justus Liebig Universitat Giessen; Alemania
dc.description.fil
Fil: Fijak, Monika. Justus Liebig Universitat Giessen; Alemania
dc.journal.title
Molecular Human Reproduction
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dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://academic.oup.com/molehr/article/13/12/853/1001866
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1093/molehr/gam067
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