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dc.contributor.author
Pigozzi, Maria Ines
dc.date.available
2021-07-16T17:56:50Z
dc.date.issued
2007-12
dc.identifier.citation
Pigozzi, Maria Ines; Localization of single-copy sequences on chicken synaptonemal complex spreads using fluorescence in situ hybridization (FISH); Karger; Cytogenetic And Genome Research; 119; 1-2; 12-2007; 105-112
dc.identifier.issn
1424-8581
dc.identifier.uri
http://hdl.handle.net/11336/136348
dc.description.abstract
Synaptonemal complex (SC) spreads from bird oocytes and spermatocytes show the complete chromosome complement and can be observed at the light microscope using immunostaining of the proteins that compose the lateral elements. To investigate the use of avian SC spreads as substrates for fluorescent in situ hybridization (FISH) in combination with immunostaining, we applied two single-copy sequences to chicken oocyte spreads. Signals for both target sequences were consistently observed on the short arm of bivalent 1 in a large number of nuclei. Based on previous data about the size of chromosome 1 and from measurements on probed SC spreads, an estimate of the physical distance in Mb between each sequence and the telomere was calculated. The crossover frequencies along SC 1 obtained by immunolocalization of MLH1 foci during pachytene were used to calculate the distances in cM to the target sequences and to compare this cytogenetic SC map with the consensus linkage map for GGA1. The combination of SC-FISH and immunostaining could be generally applied to obtain high-resolution mapping of single-copy sequences in birds and, coupled with MLH1 crossover maps, it could be a reliable approach to obtain genetic distances between markers to test the genetic linkage maps generated from molecular markers.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Karger
dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
FISH
dc.subject
SYNAPTONEMAL COMPLEX
dc.subject
MOLECULAR CYTOGENETICS
dc.subject
CHICKEN
dc.subject.classification
Genética y Herencia
dc.subject.classification
Ciencias Biológicas
dc.subject.classification
CIENCIAS NATURALES Y EXACTAS
dc.title
Localization of single-copy sequences on chicken synaptonemal complex spreads using fluorescence in situ hybridization (FISH)
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2020-09-03T17:01:29Z
dc.journal.volume
119
dc.journal.number
1-2
dc.journal.pagination
105-112
dc.journal.pais
Suiza
dc.journal.ciudad
Basel
dc.description.fil
Fil: Pigozzi, Maria Ines. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Biomédicas. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones Biomédicas; Argentina
dc.journal.title
Cytogenetic And Genome Research
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/http://content.karger.com/produktedb/produkte.asp?DOI=10.1159/000109626
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1159/000109626
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