Mostrar el registro sencillo del ítem
dc.contributor.author
de Souza, Ticiane C.
dc.contributor.author
Oliveira, Ravena Casemiro
dc.contributor.author
Bezerra, Saulo Gonçalves Santiago
dc.contributor.author
Manzo, Ricardo Martín
![Se ha confirmado la validez de este valor de autoridad por un usuario](/themes/CONICETDigital/images/authority_control/invisible.gif)
dc.contributor.author
Mammarella, Enrique José
![Se ha confirmado la validez de este valor de autoridad por un usuario](/themes/CONICETDigital/images/authority_control/invisible.gif)
dc.contributor.author
Hissa, Denise Cavalcante
dc.contributor.author
Gonçalves, Luciana R. B.
dc.date.available
2021-07-11T21:05:40Z
dc.date.issued
2021-04
dc.identifier.citation
de Souza, Ticiane C.; Oliveira, Ravena Casemiro; Bezerra, Saulo Gonçalves Santiago; Manzo, Ricardo Martín; Mammarella, Enrique José; et al.; Alternative Heterologous Expression of l-Arabinose Isomerase from Enterococcus faecium DBFIQ E36 By Residual Whey Lactose Induction; Humana Press; Molecular Biotechnology; 63; 4; 4-2021; 289-304
dc.identifier.issn
1073-6085
dc.identifier.uri
http://hdl.handle.net/11336/135803
dc.description.abstract
This study reports an alternative strategy for the expression of a recombinant l-AI from Enterococcus faecium DBFIQ E36 by auto-induction using glucose and glycerol as carbon sources and residual whey lactose as inducer agent. Commercial lactose and isopropyl β-d-1-thiogalactopyranoside (IPTG) were also evaluated as inducers for comparison of enzyme expression levels. The enzymatic extracts were purified by affinity chromatography, characterized, and applied in the bioconversion of d-galactose into d-tagatose. l-AI presented a catalytic activity of 1.67 ± 0.14, 1.52 ± 0.01, and 0.7 ± 0.04 U/mL, when expressed using commercial lactose, lactose from whey, and IPTG, respectively. Higher activities could be obtained by changing the protocol of enzyme extraction and, for instance, the enzymatic extract produced with whey presented a catalytic activity of 3.8 U/mL. The specific activity of the enzyme extracts produced using lactose (commercial or residual whey) after enzyme purification was also higher when compared to the enzyme expressed with IPTG. Best results were achieved when enzyme expression was conducted using 4 g/L of residual whey lactose for 11 h. These results proved the efficacy of an alternative and economic protocol for the effective expression of a recombinant l-AI aiming its high-scale production.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Humana Press
![Se ha confirmado la validez de este valor de autoridad por un usuario](/themes/CONICETDigital/images/authority_control/invisible.gif)
dc.rights
info:eu-repo/semantics/restrictedAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
AUTO-INDUCTION
dc.subject
CHEESE WHEY
dc.subject
D-TAGATOSE
dc.subject
ESCHERICHIA COLI EXPRESSION
dc.subject
L-ARABINOSE ISOMERASE
dc.subject.classification
Bioprocesamiento Tecnológico, Biocatálisis, Fermentación
![Se ha confirmado la validez de este valor de autoridad por un usuario](/themes/CONICETDigital/images/authority_control/invisible.gif)
dc.subject.classification
Biotecnología Industrial
![Se ha confirmado la validez de este valor de autoridad por un usuario](/themes/CONICETDigital/images/authority_control/invisible.gif)
dc.subject.classification
INGENIERÍAS Y TECNOLOGÍAS
![Se ha confirmado la validez de este valor de autoridad por un usuario](/themes/CONICETDigital/images/authority_control/invisible.gif)
dc.title
Alternative Heterologous Expression of l-Arabinose Isomerase from Enterococcus faecium DBFIQ E36 By Residual Whey Lactose Induction
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2021-07-01T17:33:22Z
dc.journal.volume
63
dc.journal.number
4
dc.journal.pagination
289-304
dc.journal.pais
Estados Unidos
![Se ha confirmado la validez de este valor de autoridad por un usuario](/themes/CONICETDigital/images/authority_control/invisible.gif)
dc.description.fil
Fil: de Souza, Ticiane C.. Universidade Estadual do Ceará; Brasil
dc.description.fil
Fil: Oliveira, Ravena Casemiro. Universidade Estadual do Ceará; Brasil
dc.description.fil
Fil: Bezerra, Saulo Gonçalves Santiago. Universidade Estadual do Ceará; Brasil
dc.description.fil
Fil: Manzo, Ricardo Martín. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Desarrollo Tecnológico para la Industria Química. Universidad Nacional del Litoral. Instituto de Desarrollo Tecnológico para la Industria Química; Argentina
dc.description.fil
Fil: Mammarella, Enrique José. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Desarrollo Tecnológico para la Industria Química. Universidad Nacional del Litoral. Instituto de Desarrollo Tecnológico para la Industria Química; Argentina
dc.description.fil
Fil: Hissa, Denise Cavalcante. Universidade Estadual do Ceará; Brasil
dc.description.fil
Fil: Gonçalves, Luciana R. B.. Universidade Estadual do Ceará; Brasil
dc.journal.title
Molecular Biotechnology
![Se ha confirmado la validez de este valor de autoridad por un usuario](/themes/CONICETDigital/images/authority_control/invisible.gif)
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1007/s12033-021-00301-2
Archivos asociados