Mostrar el registro sencillo del ítem
dc.contributor.author
Saini, Sunil Kumar
dc.contributor.author
Hersby, Ditte Stampe
dc.contributor.author
Tamhane, Tripti
dc.contributor.author
Povlsen, Helle Rus
dc.contributor.author
Amaya Hernandez, Susana Patricia
dc.contributor.author
Nielsen, Morten
![Se ha confirmado la validez de este valor de autoridad por un usuario](/themes/CONICETDigital/images/authority_control/invisible.gif)
dc.contributor.author
Gang, Anne Ortved
dc.contributor.author
Hadrup, Sine Reker
dc.date.available
2021-07-08T14:09:15Z
dc.date.issued
2021-04
dc.identifier.citation
Saini, Sunil Kumar; Hersby, Ditte Stampe; Tamhane, Tripti; Povlsen, Helle Rus; Amaya Hernandez, Susana Patricia; et al.; SARS-CoV-2 genome-wide T cell epitope mapping reveals immunodominance and substantial CD8+ T cell activation in COVID-19 patients; American Association for the Advancement of Science; Science Immunology; 6; 58; 4-2021; 1-23
dc.identifier.issn
2470-9468
dc.identifier.uri
http://hdl.handle.net/11336/135719
dc.description.abstract
T cells are important for effective viral clearance, elimination of virus-infected cells and long-term disease protection. To examine the full-spectrum of CD8+ T cell immunity in COVID-19, we experimentally evaluated 3141 major histocompatibility (MHC) class I-binding peptides covering the complete SARS-CoV-2 genome. Using DNA-barcoded peptide-MHC complex (pMHC) multimers combined with a T cell phenotype panel, we report a comprehensive list of 122 immunogenic and a subset of immunodominant SARS-CoV-2 T cell epitopes. Substantial CD8+ T cell recognition was observed in COVID-19 patients, with up to 27% of all CD8+ lymphocytes interacting with SARS-CoV-2-derived epitopes. Most immunogenic regions were derived from open reading frame (ORF) 1 and ORF3, with ORF1 containing most of the immunodominant epitopes. CD8+ T cell recognition of lower affinity was also observed in healthy donors toward SARS-CoV-2-derived epitopes. This pre-existing T cell recognition signature was partially overlapping with the epitope landscape observed in COVID-19 patients and may drive the further expansion of T cell responses to SARS-CoV-2 infection. Importantly the phenotype of the SARS-CoV-2-specific CD8+ T cells, revealed a strong T cell activation in COVID-19 patients, while minimal T cell activation was seen in healthy individuals. We found that patients with severe disease displayed significantly larger SARS-CoV-2-specific T cell populations compared to patients with mild diseases and these T cells displayed a robust activation profile. These results further our understanding of T cell immunity to SARS-CoV-2 infection and hypothesize that strong antigen-specific T cell responses are associated with different disease outcomes.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
American Association for the Advancement of Science
![Se ha confirmado la validez de este valor de autoridad por un usuario](/themes/CONICETDigital/images/authority_control/invisible.gif)
dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
Sars-Cov-2
dc.subject
Epitopes
dc.subject
T cells
dc.subject
COVID-19
dc.subject.classification
Otras Medicina Básica
![Se ha confirmado la validez de este valor de autoridad por un usuario](/themes/CONICETDigital/images/authority_control/invisible.gif)
dc.subject.classification
Medicina Básica
![Se ha confirmado la validez de este valor de autoridad por un usuario](/themes/CONICETDigital/images/authority_control/invisible.gif)
dc.subject.classification
CIENCIAS MÉDICAS Y DE LA SALUD
![Se ha confirmado la validez de este valor de autoridad por un usuario](/themes/CONICETDigital/images/authority_control/invisible.gif)
dc.title
SARS-CoV-2 genome-wide T cell epitope mapping reveals immunodominance and substantial CD8+ T cell activation in COVID-19 patients
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2021-07-07T15:15:38Z
dc.journal.volume
6
dc.journal.number
58
dc.journal.pagination
1-23
dc.journal.pais
Estados Unidos
![Se ha confirmado la validez de este valor de autoridad por un usuario](/themes/CONICETDigital/images/authority_control/invisible.gif)
dc.description.fil
Fil: Saini, Sunil Kumar. Technical University of Denmark; Dinamarca
dc.description.fil
Fil: Hersby, Ditte Stampe. Copenhagen University Hospital; Dinamarca
dc.description.fil
Fil: Tamhane, Tripti. Technical University of Denmark; Dinamarca
dc.description.fil
Fil: Povlsen, Helle Rus. Technical University of Denmark; Dinamarca
dc.description.fil
Fil: Amaya Hernandez, Susana Patricia. Technical University of Denmark; Dinamarca
dc.description.fil
Fil: Nielsen, Morten. Technical University of Denmark; Dinamarca. Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas. - Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Biotecnológicas; Argentina
dc.description.fil
Fil: Gang, Anne Ortved. Copenhagen University Hospital; Dinamarca
dc.description.fil
Fil: Hadrup, Sine Reker. Technical University of Denmark; Dinamarca
dc.journal.title
Science Immunology
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1126/sciimmunol.abf7550
Archivos asociados