Artículo
Self-collected saliva for SARS-CoV-2 detection: A prospective study in the emergency room
Echavarría, Marcela Silvia
; Reyes, Noelia Soledad; Rodriguez, Pamela Elizabeth
; Ypas, Martin; Ricarte, Joaquina Carmen
; Rodriguez, María P.; Pérez, Matías Gastón
; Seoane, Alejandro; Martinez, Alfredo; Videla, Cristina Mónica
; Stryjewski, Martin E.; Carballal, Guadalupe
Fecha de publicación:
02/02/2021
Editorial:
Wiley-liss, div John Wiley & Sons Inc.
Revista:
Journal of Medical Virology
ISSN:
0146-6615
Idioma:
Inglés
Tipo de recurso:
Artículo publicado
Clasificación temática:
Resumen
Current diagnostic standards involve severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) detection in nasopharyngeal swabs (NPS), but saliva is an attractive and noninvasive option for diagnosis. The objectives were to determine the performance of saliva in comparison with NPS for detecting SARS-CoV-2 and to compare the optimized home brew reverse-transcription polymerase chain reaction (RT-PCR) with a commercial RT-PCR. Paired NPS and saliva specimens were prospectively collected and tested by RT-PCR from patients presenting at an emergency room with signs and symptoms compatible with coronavirus disease-2019. A total of 348 samples from 174 patients were tested by RT-PCR assays. Among 174 patients with symptoms, 63 (36%) were SARS-CoV-2 positive in NPS using the optimized home-brew PCR. Of these 63 patients, 61 (98%) were also positive in saliva. An additional positive SARS-CoV-2 saliva was detected in a patient with pneumonia. Kappa Cohen´s coefficient agreement between NPS and saliva was 0.96 (95% confidence interval [CI], 0.90?0.99). Median Ct values in NPS versus saliva were 18.88 (interquartile range [IQR], 15.60?23.58; range, 11.97?38.10) versus 26.10 (IQR, 22.75?30.06; range, 13.78?39.22), respectively (p <.0001). The optimized home-brew RT-PCR demonstrated higher analytical and clinical sensitivity compared with the commercial RT-PCR assay. A high sensitivity (98%) and agreement (kappa 0.96) in saliva samples compared to NPS was demonstrated when using an optimized home-brew PCR even when the viral load in saliva was lower than in NPS. This noninvasive sample is easy to collect, requires less consumable and avoids discomfort to patients. Importantly, self-collection of saliva can diminish exposure to healthcare personnel.
Palabras clave:
COVID-19
,
NASOPHARYNGEAL SWAB
,
PCR
,
SALIVA
,
SARS-COV-2
Archivos asociados
Licencia
Identificadores
Colecciones
Articulos(CEMIC-CONICET)
Articulos de CENTRO DE EDUCACION MEDICA E INVESTIGACIONES CLINICAS "NORBERTO QUIRNO"
Articulos de CENTRO DE EDUCACION MEDICA E INVESTIGACIONES CLINICAS "NORBERTO QUIRNO"
Citación
Echavarría, Marcela Silvia; Reyes, Noelia Soledad; Rodriguez, Pamela Elizabeth; Ypas, Martin; Ricarte, Joaquina Carmen; et al.; Self-collected saliva for SARS-CoV-2 detection: A prospective study in the emergency room; Wiley-liss, div John Wiley & Sons Inc.; Journal of Medical Virology; 93; 5; 2-2-2021; 3268-3272
Compartir
Altmétricas