Artículo
Evaluation of a lyophilized crispr-cas12 assay for a sensitive, specific, and rapid detection of sars-cov-2
Curti, Lucía Ana; Primost, Ivana; Valla, Sofía Aylén
; Ibañez Alegre, Daiana Macarena
; Olguin Perglione, Cecilia; Repizo, Guillermo Daniel
; Lara, María Julia
; Parcerisa, Ivana Lorna
; Palacios, Antonela Rocio
; Llases, María Eugenia
; Rinflerch, Adriana Raquel
; Barrios, Melanie; Pereyra Bonnet, Federico Alberto
; Giménez, Carla Alejandra
; Marcone, Débora Natalia
Fecha de publicación:
05/03/2021
Editorial:
Multidisciplinary Digital Publishing Institute
Revista:
Viruses
e-ISSN:
1999-4915
Idioma:
Inglés
Tipo de recurso:
Artículo publicado
Clasificación temática:
Resumen
We evaluated a lyophilized CRISPR-Cas12 assay for SARS-CoV-2 detection (Lyo-CRISPR SARS-CoV-2 kit) based on reverse transcription, isothermal amplification, and CRISPR-Cas12 reaction. From a total of 210 RNA samples extracted from nasopharyngeal swabs using spin columns, the LyoCRISPR SARS-CoV-2 kit detected 105/105 (100%; 95% confidence interval (CI): 96.55–100) positive samples and 104/105 (99.05%; 95% CI: 94.81–99.97) negative samples that were previously tested using commercial RT-qPCR. The estimated overall Kappa index was 0.991, reflecting an almost perfect concordance level between the two diagnostic tests. An initial validation test was also performed on 30 nasopharyngeal samples collected in lysis buffer, in which the Lyo-CRISPR SARS-CoV-2 kit detected 20/21 (95.24%; 95% CI: 76.18–99.88) positive samples and 9/9 (100%; 95% CI: 66.37–100) negative samples. The estimated Kappa index was 0.923, indicating a strong concordance between the test procedures. The Lyo-CRISPR SARS-CoV-2 kit was suitable for detecting a wide range of RT-qPCR-positive samples (cycle threshold range: 11.45–36.90) and dilutions of heat-inactivated virus (range: 2.5–100 copies/µL); no cross-reaction was observed with the other respiratory pathogens tested. We demonstrated that the performance of the Lyo-CRISPR SARS-CoV-2 kit was similar to that of commercial RT-qPCR, as the former was highly sensitive and specific, timesaving (1.5 h), inexpensive, and did not require sophisticated equipment. The use of this kit would reduce the time taken for diagnosis and facilitate molecular diagnosis in low-resource laboratories.
Palabras clave:
COVID-19
,
CRISPR
,
DIAGNOSIS
,
FLUORESCENCE DETECTION
,
LYSIS BUFFER
,
SARS-COV-2
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Articulos(IBS)
Articulos de INSTITUTO DE BIOLOGIA SUBTROPICAL
Articulos de INSTITUTO DE BIOLOGIA SUBTROPICAL
Articulos(OCA HOUSSAY)
Articulos de OFICINA DE COORDINACION ADMINISTRATIVA HOUSSAY
Articulos de OFICINA DE COORDINACION ADMINISTRATIVA HOUSSAY
Citación
Curti, Lucía Ana; Primost, Ivana; Valla, Sofía Aylén; Ibañez Alegre, Daiana Macarena; Olguin Perglione, Cecilia; et al.; Evaluation of a lyophilized crispr-cas12 assay for a sensitive, specific, and rapid detection of sars-cov-2; Multidisciplinary Digital Publishing Institute; Viruses; 13; 3; 5-3-2021; 1-9
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