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dc.contributor.author
Nielsen, J. B.  
dc.contributor.author
Bagatolli, Luis Alberto  
dc.contributor.other
Humbert, P.  
dc.contributor.other
Fanian, F.  
dc.contributor.other
Maibach, H. I.  
dc.contributor.other
Agache, P.  
dc.date.available
2021-07-05T20:59:23Z  
dc.date.issued
2017  
dc.identifier.citation
Nielsen, J. B. ; Bagatolli, Luis Alberto; Storage conditions of skin affect tissue structure and in vitro percutaneus penetration; Springer International Publishing; 2017; 1191-1195  
dc.identifier.isbn
978-3-319-32381-7  
dc.identifier.uri
http://hdl.handle.net/11336/135500  
dc.description.abstract
For logistic and practical reasons, it is difficult to perform in vitro studies on percutaneous penetration on fresh human skin obtained directly from surgery. Skin samples are therefore often kept frozen until use. The present chapter presents the available literature on the topic. Storage of human skin at -20 C causes structural changes in the upper stratum corneum observable with image techniques such as multiphoton excitation fluorescence microscopy. The presently available literature does, however, not support that the observed structural damage to the integrity is sufficient to cause a general and significantly increased in vitro percutaneous penetration across human skin stored at -20 C. The use of the skin stored at -20C for in vitro studies on percutaneous penetration therefore seems acceptable as long as the barrier integrity is documented. Storage of human skin at -80 C causes significant structural changes in upper as well as deeper parts of stratum corneum. These more severe changes correspond to significantly increased percutaneous penetration of chemicals applied to skin specimens stored at very low temperatures. Storage of human skin for later use in in vitro studies on percutaneous penetration is therefore not recommended at these low temperatures  
dc.format
application/pdf  
dc.language.iso
eng  
dc.publisher
Springer International Publishing  
dc.rights
info:eu-repo/semantics/restrictedAccess  
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/  
dc.subject
SKIN  
dc.subject.classification
Biofísica  
dc.subject.classification
Ciencias Biológicas  
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CIENCIAS NATURALES Y EXACTAS  
dc.title
Storage conditions of skin affect tissue structure and in vitro percutaneus penetration  
dc.type
info:eu-repo/semantics/publishedVersion  
dc.type
info:eu-repo/semantics/bookPart  
dc.type
info:ar-repo/semantics/parte de libro  
dc.date.updated
2021-04-30T19:12:22Z  
dc.journal.pagination
1191-1195  
dc.journal.pais
Reino Unido  
dc.journal.ciudad
London  
dc.description.fil
Fil: Nielsen, J. B.. University of Southern Denmark. Department of Public Health; Dinamarca  
dc.description.fil
Fil: Bagatolli, Luis Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigación Médica Mercedes y Martín Ferreyra. Universidad Nacional de Córdoba. Instituto de Investigación Médica Mercedes y Martín Ferreyra; Argentina  
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://link.springer.com/referenceworkentry/10.1007%2F978-3-319-32383-1_47  
dc.conicet.paginas
1651  
dc.source.titulo
Agache's measuring the skin. Non-invasive investigations, physiology, normal constants  
dc.conicet.nroedicion
2