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dc.contributor.author
Cruz, Luis
dc.contributor.author
Tacken, Paul
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Bonetto, Fernando Jose
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Buschow, Sonja
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Croes, Huib
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Wijers, Miestke
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de Vries, Jolanda
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Figdor, Carl
dc.date.available
2017-03-06T17:12:48Z
dc.date.issued
2011-03
dc.identifier.citation
Cruz, Luis; Tacken, Paul; Bonetto, Fernando Jose; Buschow, Sonja; Croes, Huib; et al.; Multimodal imaging of nanovaccine carriers targeted to human dendritic cells; American Chemical Society; Molecular Pharmaceutics; 8; 3-2011; 520-531
dc.identifier.issn
1543-8384
dc.identifier.uri
http://hdl.handle.net/11336/13548
dc.description.abstract
Dendritic cells (DCs) are key players in the initiation of adaptive immune responses and are currently exploited in immunotherapy against cancer and infectious diseases. The targeted delivery of nanovaccine particles (NPs) to DCs in vivo is a promising strategy to enhance immune<br />responses. Here, targeted nanovaccine carriers were generated that allow multimodal imaging of nanocarrierDC interactions from the subcellular to the organism level. These carriers were<br />made of biodegradable poly(D,L-lactide-co-glycolide) harboring superparamagnetic iron oxide particles (SPIO) and fluorescently labeled antigen in a single particle. Targeted delivery was<br />facilitated by coating the NPs with antibodies recognizing the DC-specific receptor DC-SIGN. The fluorescent label allowed for rapid analysis and quantification of specific versus nonspecific uptake of targeted NPs by DCs compared to other blood cells. In addition, it showed that part of the encapsulated antigen reached the lysosomal compartment of DCs within 24 h. Moreover, the<br />presence of fluorescent label did not prevent the antigen from being presented to antigen-specific T cells. The incorporated SPIO was applied to track the NPs at subcellular cell organel level using transmission electron microscopy (TEM). NPs were found within endolysosomal compartments, where part of the SPIO was already released within 24 h. Furthermore, part of the NPs seemed to<br />localize within the cytoplasm. Ex vivo loading of DCs with NPs resulted in efficient labeling and detection by MRI and did not abolish cell migration within collagen scaffolds. In conclusion, incorporation of two imaging agents within a single carrier allows tracking of targeted nanovaccines on a subcellular, cellular and possibly organism level, thereby facilitating rational design of in vivo targeted vaccination strategies.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
American Chemical Society
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dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
Imaging
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Nanocarriers
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Biocompatible Materials
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Contrast Agents
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Físico-Química, Ciencia de los Polímeros, Electroquímica
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Ciencias Químicas
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CIENCIAS NATURALES Y EXACTAS
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dc.title
Multimodal imaging of nanovaccine carriers targeted to human dendritic cells
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2017-03-01T17:50:50Z
dc.journal.volume
8
dc.journal.pagination
520-531
dc.journal.pais
Estados Unidos
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dc.description.fil
Fil: Cruz, Luis. University Medical Centre; Países Bajos
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Fil: Tacken, Paul. University Medical Centre; Países Bajos
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Fil: Bonetto, Fernando Jose. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Santa Fe. Instituto de Desarrollo Tecnológico para la Industria Química (i); Argentina
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Fil: Buschow, Sonja. University Medical Centre; Países Bajos
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Fil: Croes, Huib. Radboud University Nijmegen Medical Centre; Países Bajos
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Fil: Wijers, Miestke. Radboud University Nijmegen Medical Centre; Países Bajos
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Fil: de Vries, Jolanda. University Medical Centre; Países Bajos
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Fil: Figdor, Carl. University Medical Centre; Países Bajos
dc.journal.title
Molecular Pharmaceutics
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dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/dx.doi.org/10.1021/mp100356k
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