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dc.contributor.author
Ambrosio, Rafael
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dc.contributor.author
Curatti, Leonardo
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dc.date.available
2021-07-05T16:25:04Z
dc.date.issued
2021-04
dc.identifier.citation
Ambrosio, Rafael; Curatti, Leonardo; Deferred control of ammonium cross-feeding in a N2-fixing bacterium-microalga artificial consortium; Springer; Applied Microbiology and Biotechnology; 105; 7; 4-2021; 2937-2950
dc.identifier.issn
0175-7598
dc.identifier.uri
http://hdl.handle.net/11336/135476
dc.description.abstract
There is an increasing interest in the use of N2-fixing bacteria for the sustainable biofertilization of crops. Genetically-optimized bacteria for ammonium release have an improved biofertilization capacity. Some of these strains also cross-feed ammonium into microalgae raising additional concerns on their sustainable use in agriculture due to the potential risk of producing a higher and longer-lasting eutrophication problem than synthetic N-fertilizers. Here we studied the dynamic algal cross-feeding properties of a genetically-modified Azotobacter vinelandii strain which can be tuned to over-accumulate different levels of glutamine synthetase (GS, EC 6.3.1.20) under the control of an exogenous inducer. After switching cells overaccumulating GS into a noninducing medium, they proliferated for several generations at the expense of the previously accumulated GS. Further dilution of GS by cell division slowed-down growth, promoted ammonium-excretion and cross-fed algae. The final bacterial population, and timing and magnitude of algal N-biofertlization was finely tuned in a deferred manner. This tuning was in accordance with the intensity of the previous induction of GS accumulation in the cells. This bacterial population behavior could be maintained up to about 15 bacterial cell generations, until faster-growing and nonammonium excreting cells arose at an apparent high frequency. Further improvements of this genetic engineering strategy might help to align efficiency of N-biofertilizers and safe use in an open environment.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Springer
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dc.rights
info:eu-repo/semantics/restrictedAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
AMMONIUM EXCRETION
dc.subject
EUTROPHICATION
dc.subject
NITROGEN FERTILIZER
dc.subject
NITROGEN FIXATION
dc.subject
SUSTAINABLE AGRICULTURE
dc.subject.classification
Biología Celular, Microbiología
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dc.subject.classification
Ciencias Biológicas
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dc.subject.classification
CIENCIAS NATURALES Y EXACTAS
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dc.title
Deferred control of ammonium cross-feeding in a N2-fixing bacterium-microalga artificial consortium
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2021-07-01T17:47:23Z
dc.journal.volume
105
dc.journal.number
7
dc.journal.pagination
2937-2950
dc.journal.pais
Alemania
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dc.description.fil
Fil: Ambrosio, Rafael. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones en Biodiversidad y Biotecnología; Argentina
dc.description.fil
Fil: Curatti, Leonardo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones en Biodiversidad y Biotecnología; Argentina
dc.journal.title
Applied Microbiology and Biotechnology
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dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://link.springer.com/article/10.1007/s00253-021-11210-4
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/https://doi.org/10.1007/s00253-021-11210-4
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