Mostrar el registro sencillo del ítem
dc.contributor.author
Samarfard, Samira
dc.contributor.author
McTaggart, Alistair R.
dc.contributor.author
Sharman, Murray
dc.contributor.author
Bejerman, Nicolas
dc.contributor.author
Dietzgen, Ralf G.
dc.date.available
2021-07-01T22:33:55Z
dc.date.issued
2020-03
dc.identifier.citation
Samarfard, Samira; McTaggart, Alistair R.; Sharman, Murray; Bejerman, Nicolas; Dietzgen, Ralf G.; Viromes of ten alfalfa plants in Australia reveal diverse known viruses and a novel RNA virus; MDPI AG; Pathogens; 9; 3; 3-2020; 1-24
dc.identifier.issn
2076-0817
dc.identifier.uri
http://hdl.handle.net/11336/135332
dc.description.abstract
Alfalfa plants in the field can display a range of virus-like symptoms, especially when grown over many years for seed production. Most known alfalfa viruses have RNA genomes, some of which can be detected using diagnostic assays, but many viruses of alfalfa are not well characterized. This study aims to identify the RNA and DNA virus complexes associated with alfalfa plants in Australia. To maximize the detection of RNA viruses, we purified double-stranded RNA (dsRNA) for high throughput sequencing and characterized the viromes of ten alfalfa samples that showed diverse virus-like symptoms. Using Illumina sequencing of tagged cDNA libraries from immune-captured dsRNA, we identified sequences of the single-stranded RNA viruses, alfalfa mosaic virus (AMV), bean leafroll virus, a new emaravirus tentatively named alfalfa ringspot-associated virus, and persistent dsRNA viruses belonging to the families Amalgaviridae and Partitiviridae. Furthermore, rolling circle amplification and restriction enzyme digestion revealed the complete genome of chickpea chlorosis Australia virus, a mastrevirus (family Geminiviridae) previously reported only from chickpea and French bean that was 97% identical to the chickpea isolate. The sequence data also enabled the assembly of the first complete genome (RNAs 1–3) of an Australian AMV isolate from alfalfa.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
MDPI AG
dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
alfalfa
dc.subject
virome
dc.subject
australia
dc.subject
high-throughput sequencing
dc.subject.classification
Virología
dc.subject.classification
Ciencias Biológicas
dc.subject.classification
CIENCIAS NATURALES Y EXACTAS
dc.title
Viromes of ten alfalfa plants in Australia reveal diverse known viruses and a novel RNA virus
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2021-05-11T18:13:00Z
dc.journal.volume
9
dc.journal.number
3
dc.journal.pagination
1-24
dc.journal.pais
Suiza
dc.description.fil
Fil: Samarfard, Samira. The University of Queensland; Australia
dc.description.fil
Fil: McTaggart, Alistair R.. The University of Queensland; Australia
dc.description.fil
Fil: Sharman, Murray. The University of Queensland; Australia
dc.description.fil
Fil: Bejerman, Nicolas. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigaciones Agropecuarias. Unidad de Fitopatología y Modelización Agrícola - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Unidad de Fitopatología y Modelización Agrícola; Argentina
dc.description.fil
Fil: Dietzgen, Ralf G.. The University of Queensland; Australia
dc.journal.title
Pathogens
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://www.mdpi.com/2076-0817/9/3/214
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.3390/pathogens9030214
Archivos asociados