Mostrar el registro sencillo del ítem
dc.contributor.author
Bongiovanni, Bettina
dc.contributor.author
Mata Espinosa, Dulce
dc.contributor.author
D'attilio, Luciano David
dc.contributor.author
Leon Contreras, Juan Carlos
dc.contributor.author
Marquez Velasco, Ricardo
dc.contributor.author
Bottasso, Oscar Adelmo
dc.contributor.author
Hernandez Pando, Rogelio
dc.contributor.author
Bay, Maria Luisa
dc.date.available
2017-03-03T21:00:59Z
dc.date.issued
2015-09
dc.identifier.citation
Bongiovanni, Bettina; Mata Espinosa, Dulce; D'attilio, Luciano David; Leon Contreras, Juan Carlos; Marquez Velasco, Ricardo; et al.; Effect of cortisol and/or DHEA on THP1-derived macrophages infected with Mycobacterium tuberculosis; Elsevier; Tuberculosis (edinb); 95; 5; 9-2015; 562-569
dc.identifier.issn
1472-9792
dc.identifier.uri
http://hdl.handle.net/11336/13522
dc.description.abstract
Tuberculosis (TB) is a major health problem requiring an appropriate cell immune response to be controlled. Macrophages play a central role in the response against Mycobacterium tuberculosis (Mtb).
Given our prior studies in which adrenal steroids were found to modify the cellular immune responses from TB patients, it was sensible to analyze the immunomodulatory capability of cortisol and DHEA on macrophages infected with Mtb. The human macrophage-like THP-1 cells were infected with the H37Rv strain of Mtb and treated with Cortisol and DHEA at different doses. We monitored phagocytosis, intracellular-bacterial growth, autophagosoma formation, as well as cytokine gene expression and production.
Cultures exposed to cortisol showed a decreased production of IL-1β, TNF-α, with DHEA being unable to modify the pattern of cytokine production or to reverse the cortisol inhibitory effects. Interestingly the intra-macrophagic bacterial burden was found reduced by DHEA treatment. While this effect was not related to a different cytokine pattern, in terms their production or mRNA expression, DHEA treatment did promote autophagy in Mtb-infected macrophages, irrespective of Cortisol presence.
In essence, the better control of Mtb load by DHEA-treated macrophages seems to be dependent on an autophagic mechanism. The present results are relevant for two reasons as autophagy is not only important for clearance of mycobacteria but also for the prevention of tissue damage.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Elsevier
dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
Macrophages
dc.subject
Mycobacterium Tuberculosis
dc.subject
Dehydroepiandrosterone
dc.subject
Cortisol
dc.subject
Autophagy
dc.subject.classification
Otras Ciencias Biológicas
dc.subject.classification
Ciencias Biológicas
dc.subject.classification
CIENCIAS NATURALES Y EXACTAS
dc.title
Effect of cortisol and/or DHEA on THP1-derived macrophages infected with Mycobacterium tuberculosis
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2017-02-24T19:32:55Z
dc.journal.volume
95
dc.journal.number
5
dc.journal.pagination
562-569
dc.journal.pais
Países Bajos
dc.journal.ciudad
Amsterdam
dc.description.fil
Fil: Bongiovanni, Bettina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico - CONICET - Rosario. Instituto de Inmunología Clínica y Experimental de Rosario; Argentina. Universidad Nacional de Rosario; Argentina
dc.description.fil
Fil: Mata Espinosa, Dulce. Instituto Nacional de Ciencias Médicas y Nutrición Salvador Zubirán; México
dc.description.fil
Fil: D'attilio, Luciano David. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico - CONICET - Rosario. Instituto de Inmunología Clínica y Experimental de Rosario; Argentina. Universidad Nacional de Rosario; Argentina
dc.description.fil
Fil: Leon Contreras, Juan Carlos. Instituto Nacional de Ciencias Médicas y Nutrición Salvador Zubirán; México
dc.description.fil
Fil: Marquez Velasco, Ricardo. Instituto Nacional de Cardiología Ignacio Chavez; México
dc.description.fil
Fil: Bottasso, Oscar Adelmo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico - CONICET - Rosario. Instituto de Inmunología Clínica y Experimental de Rosario; Argentina. Universidad Nacional de Rosario; Argentina
dc.description.fil
Fil: Hernandez Pando, Rogelio. Instituto Nacional de Ciencias Médicas y Nutrición Salvador Zubirán; México
dc.description.fil
Fil: Bay, Maria Luisa. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico - CONICET - Rosario. Instituto de Inmunología Clínica y Experimental de Rosario; Argentina. Universidad Nacional de Rosario; Argentina
dc.journal.title
Tuberculosis (edinb)
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1016/j.tube.2015.05.011
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S147297921530010X
Archivos asociados