Evento
Unconventional protein secretion of Helja, an apoptotic lectin
Tipo del evento:
Conferencia
Nombre del evento:
International Conference Plant Physiology & Biochemistry
Fecha del evento:
09/07/2018
Institución Organizadora:
Vienna International Science Conferences & Events Association;
Título del Libro:
International Conference Plant Physiology & Biochemistry: Programme and Abstracts
Título de la revista:
Plant Physiology & Biochemistry
Editorial:
Vienna International Science Conferences & Events Association
Idioma:
Inglés
Clasificación temática:
Resumen
Proteins following unconventional protein secretion (UPS) bypass the RE-Golgi system to reach theapoplast. They use vesicular and no vesicular routes for their transport. Vesicular UPS can encompassdifferent pathways not fully characterized. In these work we analyze the route of Helja secretion, asunflower lectin lacking signal peptide but immunodetected extracellularly. Transient expression ofrecombinant Green fluorescent Helja (GFP-Helja) in Nicotiana Benthamiana leaves allow its apoplasticobservation verified by partial co-localization (Pearson r Value= 0.65) with the specific marker sec-RFP.Florescent intracellular punctate and mobile structures were also visualized. They did not co-localizewith the Golgi marker Sialyltransferase -RFP and their distribution was not affected by Brefeldin A, theinhibitor of RE-Golgi transport.Additionally, GFP-Helja neither co-localize with the multivesicular bodies marker Rha-RFP nor with theendocytic marker FM4-64. Our results support the UPS secretion of HELJA to the apoplast by a yetunidentified vesicular pathway.
Palabras clave:
Unconventional Protein secretion
,
sunflower lectin
,
vesicles
Archivos asociados
Licencia
Identificadores
Colecciones
Eventos(IIB)
Eventos de INSTITUTO DE INVESTIGACIONES BIOLOGICAS
Eventos de INSTITUTO DE INVESTIGACIONES BIOLOGICAS
Eventos(INBIOTEC)
Eventos de INSTITUTO DE INV. EN BIODIVERSIDAD Y BIOTECNOLOGIA
Eventos de INSTITUTO DE INV. EN BIODIVERSIDAD Y BIOTECNOLOGIA
Citación
Unconventional protein secretion of Helja, an apoptotic lectin; International Conference Plant Physiology & Biochemistry; Vienna; Austria; 2018; 30-30
Compartir