Mostrar el registro sencillo del ítem
dc.contributor.author
González Arnao, María Teresa
dc.contributor.author
Hernández Ramírez, Fabiola
dc.contributor.author
Dolce, Natalia Raquel

dc.contributor.author
Rascón Díaz, Martha P.
dc.contributor.author
Cruz Cruz, Carlos A.
dc.contributor.other
Mahammad Khasim, Shaik
dc.contributor.other
Nagesh Hegde, Sadanand
dc.contributor.other
González Arnao, María T.
dc.date.available
2021-06-22T18:26:59Z
dc.date.issued
2020
dc.identifier.citation
González Arnao, María Teresa; Hernández Ramírez, Fabiola; Dolce, Natalia Raquel; Rascón Díaz, Martha P.; Cruz Cruz, Carlos A.; Cryobiotechnological Studies in Vanilla: The Orchid of Multi-industrial Uses; Springer; 2020; 21-35
dc.identifier.isbn
978-981-32-9455-4
dc.identifier.uri
http://hdl.handle.net/11336/134669
dc.description.abstract
In this work we provide new information about several cryobiotechnological studies conducted using Vanilla planifolia species as a case study. We evaluated the effect of three vitrification-based techniques, droplet-vitrification (D-V), V cryo-plate (V-Cp) and D cryo-plate (D-Cp), on the survival of two types of in vitro explants (shoot-tips and root-tips). Using differential scanning calorimetry (DSC), there were defined thermal characteristics of shoot-tips subjected to several desiccation times with SG following D-Cp procedure. It was calculated the removal of osmotically active water (OAW) by the effect of desiccation duration after the osmoprotective steps of preconditioning and loading. Using ISSR markers with six selected ISSR primers, we also evaluated the impact of tissue culture and of cryopreservation on genetic stability of vanilla shoot-tips dehydrated with the vitrification solution PVS2 and comparing D-V and V-Cp procedures. All these investigations are allowing the development and optimization of reliable cryopreservation protocols for long-term storage of Vanilla germplasm.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Springer

dc.rights
info:eu-repo/semantics/restrictedAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
CRYOPRESERVATION
dc.subject
SHOOT-TIPS
dc.subject
ROOT-TIPS
dc.subject
DSC
dc.subject
ISSR
dc.subject.classification
Otras Biotecnología Agropecuaria

dc.subject.classification
Biotecnología Agropecuaria

dc.subject.classification
CIENCIAS AGRÍCOLAS

dc.title
Cryobiotechnological Studies in Vanilla: The Orchid of Multi-industrial Uses
dc.type
info:eu-repo/semantics/publishedVersion
dc.type
info:eu-repo/semantics/bookPart
dc.type
info:ar-repo/semantics/parte de libro
dc.date.updated
2021-06-07T16:11:07Z
dc.journal.pagination
21-35
dc.journal.pais
Singapur

dc.description.fil
Fil: González Arnao, María Teresa. Universidad Veracruzana; México
dc.description.fil
Fil: Hernández Ramírez, Fabiola. Universidad Veracruzana; México
dc.description.fil
Fil: Dolce, Natalia Raquel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Botánica del Nordeste. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias. Instituto de Botánica del Nordeste; Argentina
dc.description.fil
Fil: Rascón Díaz, Martha P.. Universidad Veracruzana; México
dc.description.fil
Fil: Cruz Cruz, Carlos A.. Universidad Veracruzana; México
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://doi.org/10.1007/978-981-32-9456-1
dc.conicet.paginas
547
dc.source.titulo
Orchid Biology: Recent Trends & Challenges
Archivos asociados