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dc.contributor.author
Scremin, Oscar Umberto  
dc.contributor.author
Roch, M.  
dc.contributor.author
Norman, K.  
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Djazayeri, S.  
dc.contributor.author
Liu, Y. Y.  
dc.date.available
2017-02-24T21:38:43Z  
dc.date.issued
2015-08  
dc.identifier.citation
Scremin, Oscar Umberto; Roch, M.; Norman, K.; Djazayeri, S.; Liu, Y. Y.; Brain acetylcholine and choline concentrations and dynamics in a murine model of the Fragile X syndrome: age, sex and region-specific changes; Elsevier; Neuroscience; 301; 8-2015; 520-528  
dc.identifier.issn
0306-4522  
dc.identifier.uri
http://hdl.handle.net/11336/13410  
dc.description.abstract
Fragile X syndrome is a learning disability caused by excess of CGG repeats in the 5′ untranslated region of the Fragile X gene (FMR1) silencing its transcription and translation. We used a murine model of this condition, Fmr1 knock-out mice (KO) to study acetylcholine (ACh) metabolism and compared it to that of wild-type control mice (WT). Brain endogenous ACh (D0ACh), free choline (D0Ch), their deuterated variants D4ACh and D4Ch and mole ratios (AChMR and ChMR) were measured by gas chromatography–mass spectrometry in the cerebral hemisphere, cerebral cortex, hippocampus and cerebellum, following D4Ch administration. Regression analysis indicated a significant decrease with age (negative slope) of D4ACh, AChMR, D4Ch and ChMR in WT mice. Age dependence was only present for D4ACh and AChMR in KO mice. Analysis of variance with age as covariate indicated a significant greater D4Ch in the cerebral cortex of KO females when compared to WT females. Contrasts between sexes within genotypes indicated lower D0Ch in cortex and cerebellum of female KO mice but not in WT and lower D4Ch in hippocampus of female KO and WT mice. In conclusion, after adjusting for age, D0ACh concentrations and synthesis from deuterium-labeled Ch were similar in KO and control WT mice in all brain regions. In contrast, significant changes in Ch dynamics were found in hippocampus and cerebral cortex of KO mice that might contribute to the pathogenesis of FXS.  
dc.format
application/pdf  
dc.language.iso
eng  
dc.publisher
Elsevier  
dc.rights
info:eu-repo/semantics/openAccess  
dc.rights.uri
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/  
dc.subject
Acetylcholine Turnover  
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Mice  
dc.subject
Cholinergic Function  
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Gcms  
dc.subject
Fmr1-Ko Mice  
dc.subject.classification
Bioquímica y Biología Molecular  
dc.subject.classification
Ciencias Biológicas  
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CIENCIAS NATURALES Y EXACTAS  
dc.title
Brain acetylcholine and choline concentrations and dynamics in a murine model of the Fragile X syndrome: age, sex and region-specific changes  
dc.type
info:eu-repo/semantics/article  
dc.type
info:ar-repo/semantics/artículo  
dc.type
info:eu-repo/semantics/publishedVersion  
dc.date.updated
2017-02-23T13:55:46Z  
dc.journal.volume
301  
dc.journal.pagination
520-528  
dc.journal.pais
Países Bajos  
dc.journal.ciudad
Amsterdam  
dc.description.fil
Fil: Scremin, Oscar Umberto. Greater Los Angeles VA Healthcare System; Estados Unidos. University of California at Los Angeles; Estados Unidos. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina  
dc.description.fil
Fil: Roch, M.. Greater Los Angeles VA Healthcare System; Estados Unidos  
dc.description.fil
Fil: Norman, K.. Greater Los Angeles VA Healthcare System; Estados Unidos  
dc.description.fil
Fil: Djazayeri, S.. Greater Los Angeles VA Healthcare System; Estados Unidos  
dc.description.fil
Fil: Liu, Y. Y.. Greater Los Angeles VA Healthcare System; Estados Unidos. University of California at Los Angeles; Estados Unidos  
dc.journal.title
Neuroscience  
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1016/j.neuroscience.2015.06.036  
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0306452215005771