Mostrar el registro sencillo del ítem
dc.contributor.author
Bagatolli, Luis Alberto
![Se ha confirmado la validez de este valor de autoridad por un usuario](/themes/CONICETDigital/images/authority_control/invisible.gif)
dc.contributor.author
Parasassi, T.
dc.contributor.author
Gratton, E.
dc.date.available
2021-06-15T18:33:20Z
dc.date.issued
2000-04
dc.identifier.citation
Bagatolli, Luis Alberto; Parasassi, T.; Gratton, E.; Giant phospholipid vesicles: Comparison among the whole lipid sample characteristics using different preparation methods - A two photon fluorescence microscopy study; Elsevier Ireland; Chemistry and Physics of Lipids; 105; 2; 4-2000; 135-147
dc.identifier.issn
0009-3084
dc.identifier.uri
http://hdl.handle.net/11336/133918
dc.description.abstract
Several methods for the preparation of giant unilamellar vesicles (GUVs) using synthetic phosphatidylcholine phospholipids were evaluated. We compared the physical characteristics - in terms of lamellarity and morphology - of the whole lipid sample for each different lipid preparation using the sectioning capability of the two-photon excitation fluorescence microscope. From the evaluation of the entire lipid sample we determined that vesicle size, internal shape and shell thickness distributions depend on the vesicle's preparation method. Our results show that the preparation of giant unilamellar vesicles by the application of external electric fields offers several advantages among the other methods tested here. Using this method a high yield (~95%) of giant unilamellar vesicles with a narrow size distribution was obtained. Independently of the preparation method, some lipid structures, which are held together by lipid tethers, were identified and resolved. These particular lipid structures show shell thickness and size heterogeneity. Labeling the lipid samples with 6-lauroyl-2-(N,N- dimethylamino)naphtalene (LAURDAN) and using the LAURDAN generalized polarization function we show that the lipid packing in these tethers or tubes is similar to those found in the phospholipid vesicles. The fact that both vesicles and tethers are found in the lipid preparations indicates similar stability between these structures.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Elsevier Ireland
![Se ha confirmado la validez de este valor de autoridad por un usuario](/themes/CONICETDigital/images/authority_control/invisible.gif)
dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
6-LAUROYL-2-(N,N- DIMETHYLAMINO)NAPHTALENE
dc.subject
GENERALIZED POLARIZATION
dc.subject
GIANT UNILAMELLAR VESICLES
dc.subject
UNILAMELLAR
dc.subject.classification
Biofísica
![Se ha confirmado la validez de este valor de autoridad por un usuario](/themes/CONICETDigital/images/authority_control/invisible.gif)
dc.subject.classification
Ciencias Biológicas
![Se ha confirmado la validez de este valor de autoridad por un usuario](/themes/CONICETDigital/images/authority_control/invisible.gif)
dc.subject.classification
CIENCIAS NATURALES Y EXACTAS
![Se ha confirmado la validez de este valor de autoridad por un usuario](/themes/CONICETDigital/images/authority_control/invisible.gif)
dc.title
Giant phospholipid vesicles: Comparison among the whole lipid sample characteristics using different preparation methods - A two photon fluorescence microscopy study
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2021-04-28T20:12:56Z
dc.journal.volume
105
dc.journal.number
2
dc.journal.pagination
135-147
dc.journal.pais
Irlanda
![Se ha confirmado la validez de este valor de autoridad por un usuario](/themes/CONICETDigital/images/authority_control/invisible.gif)
dc.description.fil
Fil: Bagatolli, Luis Alberto. University of Illinois. Urbana - Champaign; Estados Unidos
dc.description.fil
Fil: Parasassi, T.. Consiglio Nazionale delle Ricerche; Italia. Istituto di Medicina Sperimentale; Italia
dc.description.fil
Fil: Gratton, E.. University of Illinois. Urbana - Champaign; Estados Unidos
dc.journal.title
Chemistry and Physics of Lipids
![Se ha confirmado la validez de este valor de autoridad por un usuario](/themes/CONICETDigital/images/authority_control/invisible.gif)
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/http://www.ncbi.nlm.nih.gov/pubmed/10823462
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1016/s0009-3084(00)00118-3
Archivos asociados