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Evento

Neutrophils from children with acute Covid-19 exhibit an inflammatory signatura with a distinct expression of high affinity receptor for IgG

Seery, VanesaIcon ; Raiden, Silvina ClaudiaIcon ; Algieri S; Di Filippo, D.; De Carli, N.; Cairoli, H.; Chiolo, M. J.; Di Lallia, S.; Grisolia, N.; Gregorio, German; Lattner, Jorge; Coll, P.; Rolon, M. J.; Melucci, C.; Sananez, InésIcon ; Holgado, P.; Ferrero, F.; Geffner, Jorge RaúlIcon ; Arruvito, L.
Colaboradores: Carrillo, Maria CristinaIcon
Tipo del evento: Reunión
Nombre del evento: LXV Reunión Anual de la Sociedad Argentina de Investigación Clínica, LXVIII Reunión Anual de la Sociedad Argentina de Inmunología y Reunión Anual de la Sociedad Argentina de Fisiología
Fecha del evento: 10/11/2020
Institución Organizadora: Sociedad Argentina de Investigación Clínica;
Título de la revista: Medicina (Buenos Aires)
Editorial: Fundación Revista Medicina
ISSN: 0025-7680
e-ISSN: 1669-9106
Idioma: Inglés
Clasificación temática:
Inmunología

Resumen

Children can be infected by severe acute respiratorysyndrome coronavirus 2 (SARS-COV-2), being most pediatriccases mild but the reason underlying are still unknown. This studyaddressed the immunopathological characteristics of neutrophilsfrom acute COVID-19 infected children.METHODS: Sixty acute COVID-19 mildly infected children (1-14years-old) and 9 healthy infants were studied. A multiparametric flowcytometry from neutrophils was performed in whole blood. Levels ofIL-1β in supernatant culture from purified neutrophils and serum antiSARS-CoV-2 IgG were quantified by ELISA.RESULTS: A low expression of CD11b (p<0.01), CD66b (p<0.01)and CD62L (p<0.001), three molecules involved in neutrophil migration,was observed in COVID-19 neutrophils (n=33) comparedwith healthy controls (n=9). Interestingly, COVID-19 neutrophilsalso showed a higher expression of two inhibitory receptors LAIR-1(p<0.01) and PECAM-1 (p<0.01). In addition, a higher expressionof two activation markers was also found in COVID-19 respect tohealthy controls: HLA-DR (p<0.0001) and the high affinity receptorfor IgG, CD64 (p<0.01). According to the functional associationbetween the β2 integrin CD11b/CD18 and receptors for IgG, wefound not only that the plasma levels of IgG antibodies directed tothe S protein of SARS-CoV-2 inversely correlated with CD11b MFI(p<0.01, r= -0.43), but also directly correlated with CD64 (p<0.05, r=0.5). CD64 is able to mediate a positive feedback on the activation ofinflammasome. Indeed, we found that neutrophils from children withCOVID-19 spontaneously produced higher concentrations of IL-1βcompared with healthy donors and increase greatly after TLR7/8 agoniststimulation.CONCLUSION: Neutrophils from infants with acute mild COVID-19have a distinct proinflammatory signature that could potentially compromisetheir ability to migrate to peripheral tissues.
Palabras clave: INFANTS , NEUTROPHILS , SARS-CoV-2 , CD11b , COVID-19
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info:eu-repo/semantics/openAccess Excepto donde se diga explícitamente, este item se publica bajo la siguiente descripción: Creative Commons Attribution-NonCommercial-ShareAlike 2.5 Unported (CC BY-NC-SA 2.5)
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URI: http://hdl.handle.net/11336/133844
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Citación
Neutrophils from children with acute Covid-19 exhibit an inflammatory signatura with a distinct expression of high affinity receptor for IgG; LXV Reunión Anual de la Sociedad Argentina de Investigación Clínica, LXVIII Reunión Anual de la Sociedad Argentina de Inmunología y Reunión Anual de la Sociedad Argentina de Fisiología; Buenos Aires; Argentina; 2020; 185-186
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