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dc.contributor.author
Garay, Fernando Sebastian
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dc.date.available
2021-06-14T15:44:03Z
dc.date.issued
2015-02
dc.identifier.citation
Garay, Fernando Sebastian; How low does the oxygen concentration go within a sandwich-type amperometric biosensor? Part 2: Theory for PPO biosensors; Elsevier Science SA; Sensors and Actuators B: Chemical; 207; 2-2015; 581-587
dc.identifier.issn
0925-4005
dc.identifier.uri
http://hdl.handle.net/11336/133801
dc.description.abstract
A mathematical model to describe the amperometric response of a sandwich-type biosensor containing polyphenol oxidase as the recognition catalytic element is proposed. The model was solved numerically and the resulting nonlinear solution was used to simulate chronoamperometric curves as well as to estimate the concentration profiles of reagents and products of the enzymatic reaction within the sensor. Fromm's systematic method was applied to get a kinetic expression for the analysis of polyphenol oxidase enzymes. The simulated data are compared with curves corresponding to a biosensor prepared with an oxidase enzyme that reacts according to a ping-pong mechanism. Although in both cases oxygen is used as mediator of the enzymatic reaction, the electrochemical step can generate it back from the H2O2 released by enzymes such as glucose oxidase but it cannot do the same in the case of working with polyphenol oxidase. Most of the calculated profiles and related data are presented using dimensioned variables so that they can be directly compared with experimental results. Relevant parameters such as limit current, response-time, and sensitivity are analyzed as function of the thickness of membranes, concentration of enzyme and concentration of substrate.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Elsevier Science SA
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dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
AMPEROMETRIC SENSOR
dc.subject
MATHEMATICAL MODELING
dc.subject
PING-PONG
dc.subject
POLYPHENOL OXIDASE
dc.subject
PPO
dc.subject
SANDWICH-TYPE BIOSENSOR
dc.subject.classification
Físico-Química, Ciencia de los Polímeros, Electroquímica
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dc.subject.classification
Ciencias Químicas
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dc.subject.classification
CIENCIAS NATURALES Y EXACTAS
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dc.title
How low does the oxygen concentration go within a sandwich-type amperometric biosensor? Part 2: Theory for PPO biosensors
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2021-04-23T18:34:29Z
dc.journal.volume
207
dc.journal.pagination
581-587
dc.journal.pais
Países Bajos
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dc.journal.ciudad
Amsterdam
dc.description.fil
Fil: Garay, Fernando Sebastian. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones en Físico-química de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Instituto de Investigaciones en Físico-química de Córdoba; Argentina
dc.journal.title
Sensors and Actuators B: Chemical
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dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1016/j.snb.2014.10.105
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/abs/pii/S0925400514013173
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