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dc.contributor.author
Caillaud, Marie-Cécile
dc.contributor.author
Piquerez, Sophie J. M.
dc.contributor.author
Fabro, Georgina
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dc.contributor.author
Steinbrenner, Jens
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Ishaque, Naveed
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Beynon, Jim
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Jones, Jonathan D. G.
dc.date.available
2021-06-14T15:41:46Z
dc.date.issued
2012-01
dc.identifier.citation
Caillaud, Marie-Cécile; Piquerez, Sophie J. M.; Fabro, Georgina; Steinbrenner, Jens; Ishaque, Naveed; et al.; Subcellular localization of the Hpa RxLR effector repertoire identifies a tonoplast-associated protein HaRxL17 that confers enhanced plant susceptibility; Wiley Blackwell Publishing, Inc; Plant Journal; 69; 2; 1-2012; 252-265
dc.identifier.issn
0960-7412
dc.identifier.uri
http://hdl.handle.net/11336/133786
dc.description.abstract
Summary Filamentous phytopathogens form sophisticated intracellular feeding structures called haustoria in plant cells. Pathogen effectors are likely to play a role in the establishment and maintenance of haustoria in addition to their better-characterized role in suppressing plant defence. However, the specific mechanisms by which these effectors promote virulence remain unclear. To address this question, we examined changes in subcellular architecture using live-cell imaging during the compatible interaction between the oomycete Hyaloperonospora arabidopsidis (Hpa) and its host Arabidopsis. We monitored host-cell restructuring of subcellular compartments within plant mesophyll cells during haustoria ontogenesis. Live-cell imaging highlighted rearrangements in plant cell membranes upon infection, in particular to the tonoplast, which was located close to the extra-haustorial membrane surrounding the haustorium. We also investigated the subcellular localization patterns of Hpa RxLR effector candidates (HaRxLs) in planta. We identified two major classes of HaRxL effector based on localization: nuclear-localized effectors and membrane-localized effectors. Further, we identified a single effector, HaRxL17, that associated with the tonoplast in uninfected cells and with membranes around haustoria, probably the extra-haustorial membrane, in infected cells. Functional analysis of selected effector candidates in planta revealed that HaRxL17 enhances plant susceptibility. The roles of subcellular changes and effector localization, with specific reference to the potential role of HaRxL17 in plant cell membrane trafficking, are discussed with respect to Hpa virulence.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Wiley Blackwell Publishing, Inc
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dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
EFFECTOR
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HAUSTORIA
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MEMBRANE
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NUCLEUS
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OOMYCETE
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RXLR
dc.subject.classification
Biología Celular, Microbiología
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dc.subject.classification
Ciencias Biológicas
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dc.subject.classification
CIENCIAS NATURALES Y EXACTAS
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dc.title
Subcellular localization of the Hpa RxLR effector repertoire identifies a tonoplast-associated protein HaRxL17 that confers enhanced plant susceptibility
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2021-04-23T16:44:54Z
dc.identifier.eissn
1365-313X
dc.journal.volume
69
dc.journal.number
2
dc.journal.pagination
252-265
dc.journal.pais
Estados Unidos
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dc.journal.ciudad
Malden
dc.description.fil
Fil: Caillaud, Marie-Cécile. John Innes Institute; Reino Unido
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Fil: Piquerez, Sophie J. M.. John Innes Institute; Reino Unido
dc.description.fil
Fil: Fabro, Georgina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Centro de Investigaciones en Química Biológica de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Centro de Investigaciones en Química Biológica de Córdoba; Argentina
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Fil: Steinbrenner, Jens. University of Warwick; Reino Unido
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Fil: Ishaque, Naveed. John Innes Institute; Reino Unido
dc.description.fil
Fil: Beynon, Jim. University of Warwick; Reino Unido
dc.description.fil
Fil: Jones, Jonathan D. G.. John Innes Institute; Reino Unido
dc.journal.title
Plant Journal
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dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://onlinelibrary.wiley.com/doi/full/10.1111/j.1365-313X.2011.04787.x
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1111/j.1365-313X.2011.04787.x
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