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Artículo

Screening methods for isolation of biocontrol epiphytic yeasts against penicillium digitatum in lemons

Pereyra, Martina MaríaIcon ; Diaz, Mariana AndreaIcon ; Soliz Santander, Fabricio Fabián; Poehlein, Anja; Meinhardt, Friedhelm; Daniel, Rolf; Dib, Julian RafaelIcon
Fecha de publicación: 03/2021
Editorial: MDPI AG
Revista: Journal of Fungi
ISSN: 2309-608X
Idioma: Inglés
Tipo de recurso: Artículo publicado
Clasificación temática:
Biología Celular, Microbiología

Resumen

Worldwide, the green rot caused by Penicillium digitatum is one of the most aggressive postharvest diseases of lemons. Searching for sustainable alternatives to chemical fungicides, epi-phytic yeasts as potential biocontrol agents were isolated from citrus fruits using a tailor-made selective medium. For disclosing their antagonistic potential against P. digitatum, obtained isolates were subjected to direct screening methods, both in vitro and in vivo. In the course of the primary in vitro screening that comprised dual culture assays, 43 yeast strains displaying antagonistic activities against the pathogen were selected. Subsequently, such strains were subjected to an in vivo screening that consisted of a microscale test, allowing the selection of six yeast strains for further analysis. In the final screening using macroscale in vivo tests, three strains (AcL2, AgL21, and AgL2) displaying the highest efficiencies to control P. digitatum were identified. The protection efficiencies in lemons were 80 (AcL2), 76.7 (AgL21), and 75% (AgL2). Based on sequence analysis of the PCR amplified D1/D2 domains of the 26S rRNA genes, they were identified as representatives of the species Clavispora lusitaniae. Interestingly, the strains exhibited a broad action spectrum among citrus fruits as they were also able to combat the green mold disease in grapefruit and two orange varieties. The direct screening methods applied in this study favored the recovery of effi-cient candidates for application as biological control agents to combat fungal infestations of citrus fruits.
Palabras clave: BIOLOGICAL CONTROL , CITRUS FRUITS , EPIPHYTIC YEASTS , PENICILLIUM DIGITATUM , SCREENING METHODS
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info:eu-repo/semantics/openAccess Excepto donde se diga explícitamente, este item se publica bajo la siguiente descripción: Creative Commons Attribution-NonCommercial-ShareAlike 2.5 Unported (CC BY-NC-SA 2.5)
Identificadores
URI: http://hdl.handle.net/11336/133779
URL: https://www.mdpi.com/2309-608X/7/3/166
DOI: https://doi.org/10.3390/jof7030166
Colecciones
Articulos(PROIMI)
Articulos de PLANTA PILOTO DE PROC.IND.MICROBIOLOGICOS (I)
Citación
Pereyra, Martina María; Diaz, Mariana Andrea; Soliz Santander, Fabricio Fabián; Poehlein, Anja; Meinhardt, Friedhelm; et al.; Screening methods for isolation of biocontrol epiphytic yeasts against penicillium digitatum in lemons; MDPI AG; Journal of Fungi; 7; 3; 3-2021; 1-13
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