Artículo
Towards efficient biocatalysts: Photo-immobilization of a lipase on novel lysozyme amyloid-like nanofibrils
Chaves, Analía Silvina; Pera, Licia Maria
; Avila, César Luis; Romero, Cintia Mariana
; Baigori, Mario Domingo
; Morán Vieyra, Eduardo; Borsarelli, Claudio Darío
; Chehin, Rosana Nieves
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Fecha de publicación:
01/2016
Editorial:
Royal Society of Chemistry
Revista:
RSC Advances
ISSN:
2046-2069
Idioma:
Inglés
Tipo de recurso:
Artículo publicado
Clasificación temática:
Resumen
Herein, we report the preparation and characterization of a new biocatalyst based on the photo-immobilization of a lipase onto hybrid protein:sugar nanofibrils. The nanofibrils are obtained through the aggregation of hen white egg lysozyme induced by the highly sulfated glycosaminoglycan heparin. The new hybrid nanomaterial could be easily functionalized using photochemical reaction to attach lipases through dityrosine covalent bonds. Compared to the free enzyme, the photo-immobilized lipase has better thermostability and enhanced resistance to non-conventional environment. Structural and morphological characterization of the nanofibrils shows that they are compatible with amyloid-like aggregates. In addition, the supramolecular arrangement of heparin and lysozyme within the building unit of the nanofibrils is also proposed. The procedure reported herein could be useful to design a new generation of insoluble biocatalyst by a single photo-click step which is definitely cleaner and faster than conventional chemical cross-linked procedures.
Palabras clave:
BIOCATALYST
,
PHOTOIMMOBILIZATION
,
AMYLOID-LIKE NANOFIBRILS
,
LIPASE
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Licencia
Identificadores
Colecciones
Articulos(PROIMI)
Articulos de PLANTA PILOTO DE PROC.IND.MICROBIOLOGICOS (I)
Articulos de PLANTA PILOTO DE PROC.IND.MICROBIOLOGICOS (I)
Citación
Chaves, Analía Silvina; Pera, Licia Maria; Avila, César Luis; Romero, Cintia Mariana; Baigori, Mario Domingo; et al.; Towards efficient biocatalysts: Photo-immobilization of a lipase on novel lysozyme amyloid-like nanofibrils; Royal Society of Chemistry; RSC Advances; 6; 11; 1-2016; 8528-8538
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