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dc.contributor.author
Demonte, Ana M.
dc.contributor.author
Asención Diez, Matías Damián
dc.contributor.author
Guerrero, Sergio Adrian
dc.contributor.author
Ballicora, Miguel A.
dc.contributor.author
Iglesias, Alberto Alvaro
dc.date.available
2017-02-24T14:59:51Z
dc.date.issued
2014-09
dc.identifier.citation
Demonte, Ana M.; Asención Diez, Matías Damián; Guerrero, Sergio Adrian; Ballicora, Miguel A.; Iglesias, Alberto Alvaro; Iodine Staining of Escherichia coli Expressing Genes Involved in the Synthesis of Bacterial Glycogen; BioProtocol Inc.; Bio-protocol; 4; 17; 9-2014; 2558
dc.identifier.issn
2331-8325
dc.identifier.uri
http://hdl.handle.net/11336/13367
dc.description.abstract
The presence of intracellular glycogen can be detected by the following iodine staining technique. Cells with glycogen stain dark brown, whereas in its absence they remain with a pale yellowish color. It is hypothesized that iodine atoms fit into helical coils formed by the α-polyglucan to form a coloured glycogen-iodine complex. Here, we have studied the expression of Streptococcus mutans (S. mutans) genes that control the biosynthesis of this polysaccharide. Thus, we expressed glgC and glgD genes coding for both ADP-Glc pyrophosphorylase subunits in Escherichia coli (E. coli) AC70R1-504 cells to complement the deficient accumulation of glycogen by this strain. In control cells or in those where an inactive protein was expressed, the synthesis of the polysaccharide was undetectable by this iodine staining technique.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
BioProtocol Inc.
dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
Polysaccharide Detection
dc.subject
Bacteria Carbohydrate
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Glycogen Metabolism
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Polyglucan Storage
dc.subject.classification
Bioquímica y Biología Molecular
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Ciencias Biológicas
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CIENCIAS NATURALES Y EXACTAS
dc.title
Iodine Staining of Escherichia coli Expressing Genes Involved in the Synthesis of Bacterial Glycogen
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2016-11-23T20:13:00Z
dc.journal.volume
4
dc.journal.number
17
dc.journal.pagination
2558
dc.journal.pais
Estados Unidos
dc.description.fil
Fil: Demonte, Ana M.. Universidad Nacional del Litoral; Argentina
dc.description.fil
Fil: Asención Diez, Matías Damián. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Santa Fe. Instituto de Agrobiotecnologia del Litoral; Argentina. Universidad Nacional del Litoral; Argentina
dc.description.fil
Fil: Guerrero, Sergio Adrian. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Santa Fe. Instituto de Agrobiotecnologia del Litoral; Argentina
dc.description.fil
Fil: Ballicora, Miguel A.. Loyola University Chicago. Department of Chemistry & Biochemistry; Estados Unidos
dc.description.fil
Fil: Iglesias, Alberto Alvaro. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Santa Fe. Instituto de Agrobiotecnologia del Litoral; Argentina. Universidad Nacional del Litoral; Argentina
dc.journal.title
Bio-protocol
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/http://www.bio-protocol.org/e1224
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://doi.org/10.21769/BioProtoc.1224
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