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dc.contributor.author
Mazaira, Gisela Ileana
dc.contributor.author
Echeverria, Pablo C.
dc.contributor.author
Galigniana, Mario Daniel
dc.date.available
2021-06-11T13:06:01Z
dc.date.issued
2020-06
dc.identifier.citation
Mazaira, Gisela Ileana; Echeverria, Pablo C.; Galigniana, Mario Daniel; Nucleocytoplasmic shuttling of the glucocorticoid receptor is influenced by tetratricopeptide repeat containing proteins; Company of Biologists; Journal of Cell Science; 133; 12; 6-2020; 1-13
dc.identifier.issn
0021-9533
dc.identifier.uri
http://hdl.handle.net/11336/133670
dc.description.abstract
It has been demonstrated that tetratricopeptide-repeat (TPR) domain proteins regulate the subcellular localization of glucocorticoid receptor (GR). This study analyses the influence of the TPR domain of high molecular weight immunophilins in the retrograde transport and nuclear retention of GR. Overexpression of the TPR peptide prevented efficient nuclear accumulation of the GR by disrupting the formation of complexes with the dynein-associated immunophilin FKBP52 (also known as FKBP4), the adaptor transporter importin-β1 (KPNB1), the nuclear pore-associated glycoproteinNup62 and nuclear matrix-associated structures. We also show that nuclear import of GR was impaired, whereas GR nuclear export was enhanced. Interestingly, the CRM1 (exportin-1) inhibitor leptomycin-B abolished the effects of TPR peptide overexpression, although the drug did not inhibit GR nuclear export itself. This indicates the existence of a TPRdomain-dependent mechanism for the export of nuclear proteins. The expression balance of those TPR domain proteins bound to the GR-Hsp90 complex may determine the subcellular localization and nucleocytoplasmic properties of the receptor, and thereby its pleiotropic biological properties in different tissues and cell types.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Company of Biologists
dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
FKBP52
dc.subject
GLUCOCORTICOID RECEPTOR
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HSP90
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IMMUNOPHILINS
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NUCLEAR MATRIX
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TETRATRICOPEPTIDE REPEATS
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Bioquímica y Biología Molecular
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Medicina Básica
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CIENCIAS MÉDICAS Y DE LA SALUD
dc.title
Nucleocytoplasmic shuttling of the glucocorticoid receptor is influenced by tetratricopeptide repeat containing proteins
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2021-06-07T16:57:04Z
dc.journal.volume
133
dc.journal.number
12
dc.journal.pagination
1-13
dc.journal.pais
Reino Unido
dc.journal.ciudad
Cambridge
dc.description.fil
Fil: Mazaira, Gisela Ileana. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Biológica; Argentina
dc.description.fil
Fil: Echeverria, Pablo C.. Universite de Geneve. Department Of Earth Sciences.; Suiza
dc.description.fil
Fil: Galigniana, Mario Daniel. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina
dc.journal.title
Journal of Cell Science
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/http://jcs.biologists.org/lookup/doi/10.1242/jcs.238873
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1242/jcs.238873
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