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Artículo

Genetic dissection of a motility-associated c-di-GMP signalling protein of Pseudomonas putida

Österberg, Sofia ; Åberg, Anna ; Herrera Seitz, KarinaIcon ; Wolf Watz, Magnus ; Shingler, Victoria
Fecha de publicación: 08/2013
Editorial: Wiley
Revista: Environmental Microbiology Reports
e-ISSN: 1758-2229
Idioma: Inglés
Tipo de recurso: Artículo publicado
Clasificación temática:
Biología Celular, Microbiología

Resumen

Lack of the Pseudomonas putida PP2258 protein or its overexpression results in defective motility on solid media. The PP2258 protein is tripartite, possessing a PAS domain linked to two domains associated with turnover of c-di-GMP – a cyclic nucleotide that controls the switch between motile and sessile lifestyles. The second messenger c-di-GMP is produced by diguanylate cyclases and degraded by phosphodiesterases containing GGDEF and EAL or HD-GYP domains respectively. It is common for enzymes involved in c-di-GMP signalling to contain two domains with potentially opposing c-di-GMP turnover activities; however, usually one is degenerate and has been adopted to serve regulatory functions. Only a few proteins have previously been found to have dual enzymatic activities – being capable of both synthesizing and hydrolysing c-di-GMP. Here, using truncated and mutant derivatives of PP2258, we show that despite a lack of complete consensus in either the GGDEF or EAL motifs, the two c-di-GMP turnover domains can function independently of each other, and that the diguanylate cyclase activity is regulated by an inhibitory I-site within its GGDEF domain. Thus, motility-associated PP2258 can be added to the short list of bifunctional c-di-GMP signalling proteins.
Palabras clave: Chemotaxis , Pseudomonas Putida , C-Di-Gmp
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info:eu-repo/semantics/openAccess Excepto donde se diga explícitamente, este item se publica bajo la siguiente descripción: Creative Commons Attribution-NonCommercial-ShareAlike 2.5 Unported (CC BY-NC-SA 2.5)
Identificadores
URI: http://hdl.handle.net/11336/13344
URL: http://onlinelibrary.wiley.com/doi/10.1111/1758-2229.12045/abstract
URL: http://dx.doi.org/10.1111/1758-2229.12045
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Articulos(IIB)
Articulos de INSTITUTO DE INVESTIGACIONES BIOLOGICAS
Citación
Österberg, Sofia ; Åberg, Anna ; Herrera Seitz, Karina; Wolf Watz, Magnus ; Shingler, Victoria ; Genetic dissection of a motility-associated c-di-GMP signalling protein of Pseudomonas putida; Wiley; Environmental Microbiology Reports; 5; 4; 8-2013; 556-565
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