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dc.contributor.author
Ferrulli, Mariana
dc.contributor.author
Pérez Rojo, Fernando Gabriel
dc.contributor.author
Granada Herrera, Lilian Andrea
dc.contributor.author
Maglioco, Andrea Florencia
dc.contributor.author
Roldán, Emilio A. J.
dc.contributor.author
Fuchs, Alicia Graciela
dc.date.available
2021-06-07T03:46:05Z
dc.date.issued
2019-02
dc.identifier.citation
Ferrulli, Mariana; Pérez Rojo, Fernando Gabriel; Granada Herrera, Lilian Andrea; Maglioco, Andrea Florencia; Roldán, Emilio A. J.; et al.; Effect of Etidronate and Ibandronate on Cytosolic Ca2+ in HT29 and Parasite Cell Line from Echinococcus Granulosus sensu lato; Bentham Science Publishers; The Open Parasitology Journal; 7; 1; 2-2019; 19-25
dc.identifier.issn
1874-4214
dc.identifier.uri
http://hdl.handle.net/11336/133289
dc.description.abstract
Background: The bisphosphonates are synthetic analogs of pyrophosphate in which two phosphates are connected through carbon instead of oxygen. They are approved compounds for the treatment of hypercalcemia, bone diseases and they have been proposed to treat infectious diseases. Bisphosphonates’ main mechanisms of action are on calcium metabolism, inhibition of protein prenylation and on ATP synthesis. In a previous work, the antiparasitic activity of bisphosphonates on a cell line from Echinococcus granulosus, sensu lato protoscoleces, 30 µM etidronate and ibandronate have antiproliferative activity after 72 h of incubation, decreasing intracellular ATP and only etidronate increased intracellular total calcium concentration. Objective: This work studied the effect of etidronate and ibandronate on cytoplasmic ionic calcium concentration in parasitic cell line and in HT29, cell line from human colon adenocarcinoma. Methods: Ionic calcium was measured by spectrofluorometric, labeling cells with Fluo-4AM. Cells were suspended in Na+ or K+ rich buffer and two calcium salts were used Cl- or Gluc- , anion permeable and impermeable, respectively. Results: Remarkable differences between cell lines were shown with the effect of bisphosphonates on intracellular ionic calcium concentration in hyperpolarized cells and these differences were smoothed on depolarized cells, in spite of the similar cellular response to calcium salts in absence of bisphosphonates. Conclusion: The bisphosphonates, mainly etidronate, decreased intracellular ionic calcium on parasitic cells explaining other aspects of their antiproliferative effect. Results suggested that other mechanism, such as Cl- and Na+ interchange are differentially affected by bisphosphonates, depending on cell line origin
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Bentham Science Publishers
dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by/2.5/ar/
dc.subject
CALCIUM SALTS
dc.subject
CYTOPLASMIC CALCIUM
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ECHINOCOCCUS GRANULOSUS
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ETIDRONATE
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HT29 CELL
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IBANDRONATE
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Farmacología y Farmacia
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Medicina Básica
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CIENCIAS MÉDICAS Y DE LA SALUD
dc.title
Effect of Etidronate and Ibandronate on Cytosolic Ca2+ in HT29 and Parasite Cell Line from Echinococcus Granulosus sensu lato
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2020-11-09T19:28:37Z
dc.journal.volume
7
dc.journal.number
1
dc.journal.pagination
19-25
dc.journal.pais
Países Bajos
dc.description.fil
Fil: Ferrulli, Mariana. Universidad Abierta Interamericana. Secretaría de Investigación. Centro de Altos Estudios En Ciencias Humanas y de la Salud - Sede Buenos Aires; Argentina
dc.description.fil
Fil: Pérez Rojo, Fernando Gabriel. Universidad Abierta Interamericana. Secretaría de Investigación. Centro de Altos Estudios En Ciencias Humanas y de la Salud - Sede Buenos Aires; Argentina
dc.description.fil
Fil: Granada Herrera, Lilian Andrea. Universidad Abierta Interamericana. Secretaría de Investigación. Centro de Altos Estudios En Ciencias Humanas y de la Salud - Sede Buenos Aires; Argentina
dc.description.fil
Fil: Maglioco, Andrea Florencia. Universidad Abierta Interamericana. Secretaría de Investigación. Centro de Altos Estudios En Ciencias Humanas y de la Salud - Sede Buenos Aires; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
dc.description.fil
Fil: Roldán, Emilio A. J.. Universidad Abierta Interamericana. Secretaría de Investigación. Centro de Altos Estudios En Ciencias Humanas y de la Salud - Sede Buenos Aires; Argentina
dc.description.fil
Fil: Fuchs, Alicia Graciela. Universidad Abierta Interamericana. Secretaría de Investigación. Centro de Altos Estudios En Ciencias Humanas y de la Salud - Sede Buenos Aires; Argentina. Dirección Nacional de Instituto de Investigación. Administración Nacional de Laboratorio e Instituto de Salud "Dr. C. G. Malbrán". Instituto Nacional de Parasitología "Dr. Mario Fatala Chaben"; Argentina
dc.journal.title
The Open Parasitology Journal
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://openparasitologyjournal.com/VOLUME/7/PAGE/19/
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.2174/1874421401907010019
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