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dc.contributor.author
Marson, María Elena
dc.contributor.author
Bournissen, Facundo García
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Altcheh, Jaime Marcelo
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Moscatelli, Guillermo
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Moroni, Samantha
dc.contributor.author
Mastrantonio Garrido, Guido Enrique
dc.date.available
2021-06-03T03:49:53Z
dc.date.issued
2020
dc.identifier.citation
Marson, María Elena; Bournissen, Facundo García; Altcheh, Jaime Marcelo; Moscatelli, Guillermo; Moroni, Samantha; et al.; Presence of benznidazole conjugated metabolites in urine identified by β-glucuronidase treatment; Universidade de São Paulo; Brazilian Journal Of Pharmaceutical Sciences; 56; e18034; 2020; 1-8
dc.identifier.uri
http://hdl.handle.net/11336/133066
dc.description.abstract
Chagas disease is a serious public health problem in Latin America and, due to migration, in other non-endemic regions. Benznidazole (BNZ) is first choice drug in pediatric therapeutics. However, little is known regarding its metabolism in humans. The aim of the study was to isolate and identify products of human BZN metabolism in urine samples obtained from a pediatric Chagas patient and a healthy adult volunteer both treated with BZN. Urine samples were collected after dose of BNZ. Urine was treated with β-glucuronidase followed by an extraction procedure under two different pH conditions and a HPLC/UV and MS/MS identification of BZN and its metabolites. BZN (m/z 260.09847) was identified in all urine extracts. Peaks from each extracted chromatograms were selected for MS and MS/MS identification. Three compounds structurally related to BZN were identified: BZN-Na+ (m/z 283.08009), N-amine-BZN (m/z 230.12307) and N-hydroxi-amine-BZN (m/z 246.11702). BNZ-Na+ was identified in all extracts, but N-amine-BZN and N-hydroxi-amine-BZN were only observed in those extracts treated with β-glucuronidase. This is the first experimental report showing elimination of BZN N-reduced metabolites in urine. As they were released after treatment with β-glucuronidase it can be suggested that glucuronization plays a role in BNZ metabolism and renal elimination.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Universidade de São Paulo
dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by/2.5/ar/
dc.subject
ANTIPARASITIC AGENTS/PHARMACOLOGY
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BENZNIDAZOLE METABOLITES
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CHAGAS DISEASE/DRUG THERAPY
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GLUCURONIDASE/URINE
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HIGH PERFORMANCE LIQUID CHROMATOGRAPHY HPLC/METHODS
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MASS SPECTROMETRY/ METHODS
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Enfermedades Infecciosas
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Ciencias de la Salud
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CIENCIAS MÉDICAS Y DE LA SALUD
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Química Analítica
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Ciencias Químicas
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CIENCIAS NATURALES Y EXACTAS
dc.title
Presence of benznidazole conjugated metabolites in urine identified by β-glucuronidase treatment
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2021-05-28T16:01:32Z
dc.identifier.eissn
2175-9790
dc.journal.volume
56
dc.journal.number
e18034
dc.journal.pagination
1-8
dc.journal.pais
Brasil
dc.journal.ciudad
San Pablo
dc.description.fil
Fil: Marson, María Elena. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Departamento de Ciencias Biológicas. Área de Toxicología; Argentina. Comision de Investigaciones Cientificas de la Provincia de Buenos Aires. Unidad Plapimu Laseisic.; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; Argentina
dc.description.fil
Fil: Bournissen, Facundo García. Gobierno de la Ciudad de Buenos Aires. Hospital General de Niños "Ricardo Gutiérrez". Servicio de Parasitología y Chagas; Argentina
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Fil: Altcheh, Jaime Marcelo. Gobierno de la Ciudad de Buenos Aires. Hospital General de Niños "Ricardo Gutiérrez". Servicio de Parasitología y Chagas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
dc.description.fil
Fil: Moscatelli, Guillermo. Gobierno de la Ciudad de Buenos Aires. Hospital General de Niños "Ricardo Gutiérrez". Servicio de Parasitología y Chagas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
dc.description.fil
Fil: Moroni, Samantha. Gobierno de la Ciudad de Buenos Aires. Hospital General de Niños "Ricardo Gutiérrez". Servicio de Parasitología y Chagas; Argentina
dc.description.fil
Fil: Mastrantonio Garrido, Guido Enrique. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Departamento de Ciencias Biológicas. Área de Toxicología; Argentina. Comision de Investigaciones Cientificas de la Provincia de Buenos Aires. Unidad Plapimu Laseisic.; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; Argentina
dc.journal.title
Brazilian Journal Of Pharmaceutical Sciences
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502020000100520&tlng=en
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1590/s2175-97902019000218034
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