Evento
Vasoactive intestinal peptide (vip) as an ovarian protector: prevention against premature ovarian failure during chemotherapy
Herrero, Yamila; Scotti, Leopoldina
; Oubiña, Gonzalo
; Pascuali, Natalia Marisa
; Ramhorst, Rosanna Elizabeth
; Perez Leiros, Claudia
; Abramovich, Dalhia Nurit
; Parborell, Maria Fernanda Agustina
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Tipo del evento:
Reunión
Nombre del evento:
LXIV Reunión Anual de la Sociedad Argentina de Investigación Clínica; LI Reunión Anual de la Asociación Argentina de Farmacología Experimenta; XXI Reunión Anual de la Sociedad Argentina de Biología; XXXI Reunión Anual de la Sociedad Argentina de Protozoología; IX Reunión Anual de la Asociación Argentina de Nanomedicinas y VI Reunión Científica Regional de la Asociación Argentina de Ciencia y Tecnología de Animales de Laboratorio
Fecha del evento:
13/11/2019
Institución Organizadora:
Sociedad Argentina de Investigación Clínica;
Asociación Argentina de Farmacología Experimental;
Sociedad Argentina de Biología;
Sociedad Argentina de Protozoología;
Asociación Argentina de Nanomedicinas;
Asociación Argentina de Ciencia y Tecnología de Animales de Laboratorio;
The Histochemical Society;
Título de la revista:
Medicina (Buenos Aires)
Editorial:
Fundación Revista Medicina
ISSN:
0025-7680
e-ISSN:
1669-9106
Idioma:
Inglés
Clasificación temática:
Resumen
The ovary, in addition to its endocrine and intraovarian control, is regulated by direct neural inputs of peptidergic nature. Vasoactive intestinal peptide (VIP) was originally isolated from the small intestine and lung tissues and plays an important role in ovarian function. Previous studies have found VIP immunoreactivity in ovarian follicles. The objective of this study was to determine the effect of VIP on ovarian function in a doxorubicin (DX) induced-Premature Ovarian Failure (POF) murine model.To induce POF, DX 10mg/kg, i.p. was applied in F1 mice (C57XBalbC 8 weeks old) on day 1. Control and DX mice underwent sham surgery and received an intrabursal injection of saline solution on both ovaries, while DX + VIP groups received either 1μl or 10µl VIP 1µM 1 h prior to DX administration. Sacrifices were made at day 15. The ovaries were isolated for histological analysis and protein extraction for Western Blot assays. For all data analysis ANOVA followed by Tukey test were performed. An ovarian morphological analysis showed that DX decreased the % of primary (PriF), preantral (PF) and early antral follicles (EAF), and increased the % of atretic follicles (AtrF) (p<0.05). VIP (1 µl) increased the % of EAF and decreased the % AtrF. However, the highest dose of VIP (10 µl) increased the % of PriF, PF and EAF, and decreased the % of AtrF compared to DX (p<0.05). These results were corroborated by IHC for Anti-Müllerian Hormone (AMH), where it was found that DX reduced the % of follicles expressing AMH, while VIP (both doses) increased it (p<0.001). DX increased the apoptotic index (cleaved caspase-3-positive follicles/total follicles) in follicles, compared to control (P<0.01). VIP (both doses) protected follicles from this increment.In conclusion, VIP might be a promising strategy to protect female fertility in cancer patients. Further studies on VIP effects on female reproduction in chemotherapy-induced POF and on the safety of use of this peptide are required.
Palabras clave:
OVARY
,
CHEMOTHERAPY
,
VIP
Archivos asociados
Licencia
Identificadores
Colecciones
Eventos(IBYME)
Eventos de INST.DE BIOLOGIA Y MEDICINA EXPERIMENTAL (I)
Eventos de INST.DE BIOLOGIA Y MEDICINA EXPERIMENTAL (I)
Eventos(IQUIBICEN)
Eventos de INSTITUTO DE QUIMICA BIOLOGICA DE LA FACULTAD DE CS. EXACTAS Y NATURALES
Eventos de INSTITUTO DE QUIMICA BIOLOGICA DE LA FACULTAD DE CS. EXACTAS Y NATURALES
Citación
Vasoactive intestinal peptide (vip) as an ovarian protector: prevention against premature ovarian failure during chemotherapy; LXIV Reunión Anual de la Sociedad Argentina de Investigación Clínica; LI Reunión Anual de la Asociación Argentina de Farmacología Experimenta; XXI Reunión Anual de la Sociedad Argentina de Biología; XXXI Reunión Anual de la Sociedad Argentina de Protozoología; IX Reunión Anual de la Asociación Argentina de Nanomedicinas y VI Reunión Científica Regional de la Asociación Argentina de Ciencia y Tecnología de Animales de Laboratorio; Mar del Plata; Argentina; 2019; 292
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