Artículo
Muscarinic inhibition of hippocampal and striatal adenylyl cyclase is mainly due to the m4 receptor
Sánchez, Gonzalo Manuel; Colettis, Natalia Claudia
; Vazquez, Pablo
; Cerveñansky, Carlos; Aguirre, Alejandra Inés
; Quillfeldt, Jorge A.; Jerusalinsky, Diana Alicia
; Kornisiuk, Edgar Ernesto
Fecha de publicación:
08/2009
Editorial:
Springer/Plenum Publishers
Revista:
Neurochemical Research
ISSN:
0364-3190
Idioma:
Inglés
Tipo de recurso:
Artículo publicado
Clasificación temática:
Resumen
The five muscarinic acetylcholine receptors (M(1)-M(5)) are differentially expressed in the brain. M(2) and M(4) are coupled to inhibition of stimulated adenylyl cyclase, while M(1), M(3) and M(5) are mainly coupled to the phosphoinositide pathway. We studied the muscarinic receptor regulation of adenylyl cyclase activity in the rat hippocampus, compared to the striatum and amygdala. Basal and forskolin-stimulated adenylyl cyclase activity was higher in the striatum but the muscarinic inhibition was much lower. Highly selective muscarinic toxins MT1 and MT2-affinity order M(1) > or = M(4) >> others-and MT3-highly selective M(4) antagonist-did not show significant effects on basal or forskolin-stimulated cyclic AMP production but, like scopolamine, counteracted oxotremorine inhibition. Since MTs have negligible affinity for M(2), M(4) would be the main subtype responsible for muscarinic inhibition of forskolin-stimulated enzyme. Dopamine stimulated a small fraction of the enzyme (3.1% in striatum, 1.3% in the hippocampus). Since MT3 fully blocked muscarinic inhibition of dopamine-stimulated enzyme, M(4) receptor would be responsible for this regulation.
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Articulos(IBCN)
Articulos de INST.DE BIOLO.CEL.Y NEURCS."PROF.E.DE ROBERTIS"
Articulos de INST.DE BIOLO.CEL.Y NEURCS."PROF.E.DE ROBERTIS"
Citación
Sánchez, Gonzalo Manuel; Colettis, Natalia Claudia; Vazquez, Pablo; Cerveñansky, Carlos; Aguirre, Alejandra Inés; et al.; Muscarinic inhibition of hippocampal and striatal adenylyl cyclase is mainly due to the m4 receptor; Springer/Plenum Publishers; Neurochemical Research; 34; 8; 8-2009; 1363-1371
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