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Artículo

Isolation and characterization of a Solanum tuberosum subtilisin-like protein with caspase- 3 activity (StSBTc-3)

Fernández, María BelénIcon ; Daleo, Gustavo RaulIcon ; Guevara, Maria GabrielaIcon
Fecha de publicación: 03/12/2014
Editorial: Elsevier Masson
Revista: Plant Physiology And Biochemistry
ISSN: 0981-9428
Idioma: Inglés
Tipo de recurso: Artículo publicado
Clasificación temática:
Bioquímica y Biología Molecular

Resumen

Plant proteases with caspase-like enzymatic activity have been widely studied during the last decade. Previously, we have reported the presence and induction of caspase-3 like activity in the apoplast of potato leaves during Solanum tuberosum- Phytophthora infestans interaction. In this work we have purified and identified a potato extracellular protease with caspase-3 like enzymatic activity from potato leaves infected with P. infestans. Results obtained from the size exclusion chromatography show that the isolated protease is a monomeric enzyme with an estimated molecular weight of 70 kDa approximately. Purified protease was analyzed by MALDI-TOF MS, showing a 100% of sequence identity with the deduced amino acid sequence of a putative subtilisin-like protease from S. tuberosum (Solgenomics protein ID: PGSC0003DMP400018521). For this reason the isolated protease was named as StSBTc-3. This report constitutes the first evidence of isolation and identification of a plant subtilisin-like protease with caspase-3 like enzymatic activity. In order to elucidate the possible function of StSBTc-3 during plant pathogen interaction, we demonstrate that like animal caspase-3, StSBTc-3 is able to produce in vitro cytoplasm shrinkage in plant cells and to induce plant cell death. This result suggest that, StSBTc-3 could exert a caspase executer function during potato- P. infestans interaction, resulting in the restriction of the pathogen spread during plant–pathogen interaction.
Palabras clave: Solanum Tuberosum , Subtilisin Like Protein , Devdase , Apoplast , Plant-Pathogen Interaction
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info:eu-repo/semantics/openAccess Excepto donde se diga explícitamente, este item se publica bajo la siguiente descripción: Atribución-NoComercial-SinDerivadas 2.5 Argentina (CC BY-NC-ND 2.5 AR)
Identificadores
URI: http://hdl.handle.net/11336/13280
URL: http://www.sciencedirect.com/science/article/pii/S0981942814003647
DOI: http://dx.doi.org/10.1016/j.plaphy.2014.12.001
Colecciones
Articulos(IIB)
Articulos de INSTITUTO DE INVESTIGACIONES BIOLOGICAS
Citación
Fernández, María Belén; Daleo, Gustavo Raul; Guevara, Maria Gabriela; Isolation and characterization of a Solanum tuberosum subtilisin-like protein with caspase- 3 activity (StSBTc-3); Elsevier Masson; Plant Physiology And Biochemistry; 86; 03-12-2014; 137-146
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