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dc.contributor.author
Ferrante, Alejandro Antonio  
dc.contributor.author
Baca Castex, Clara  
dc.contributor.author
Bruno, Santiago Rafael  
dc.contributor.author
Arraztoa, Claudia Cecilia  
dc.contributor.author
Plaza, Jessica Paula  
dc.contributor.author
Neild, Deborah  
dc.contributor.author
Miragaya, Marcelo  
dc.date.available
2021-05-28T15:11:50Z  
dc.date.issued
2018-06  
dc.identifier.citation
Ferrante, Alejandro Antonio; Baca Castex, Clara; Bruno, Santiago Rafael; Arraztoa, Claudia Cecilia; Plaza, Jessica Paula; et al.; Comparison of whole and centrifuged egg-yolk added to Kenney´s and Lactose-EDTA extenders for donkey semen cryopreservation; Elsevier Science Inc; Journal of Equine Veterinary Science; 65; 6-2018; 12-18  
dc.identifier.issn
0737-0806  
dc.identifier.uri
http://hdl.handle.net/11336/132740  
dc.description.abstract
Egg yolk (EY) has been the most widely used external cryoprotectant for donkey spermatozoa; however, it interferes with semen evaluation. Using clarified EY in equine freezing extenders has been successful, but it is difficult to apply in mountainous areas where mules are bred. Thus, the objective of this study was to evaluate the use of centrifuged EY for deep-freezing donkey spermatozoa. Twelve ejaculates were collected from four jacks of proven fertility. Seminal plasma was removed and, before freezing, samples were rediluted in four extenders, each with 7% dimethylformamide (DMF): (1) Kenney extender with 20% EY (Kenney-Y); (2) Kenney extender with 20% centrifuged EY (Kenney-CY); (3) EDTA-glucose extender with 20% EY (EDTA-Y); and (4) EDTA-glucose extender with 20% centrifuged EY (EDTA-CY). After thawing, motility, membrane function, and acrosome status were evaluated. Total and progressive motility observed in Kenney-Y and EDTA-Y was higher (P <.05) than that in Kenney-CY and EDTA-CY. The percentage of frozen-thawed sperm with functional membranes and intact acrosomes was higher (P <.05) in the samples extended with EY when compared with those extended with centrifuged EY. In addition, samples extended with centrifuged EY showed a higher (P <.05) proportion of sperm with nonfunctional membranes and percent of detached acrosomes. This study has shown that it is possible to freeze donkey semen with 7% DMF using a rapid curve of temperature decrease and a manual freezing procedure. However, addition of centrifuged EY to semen freezing extenders had an unexpected detrimental effect on sperm motility, membrane function, and acrosome status.  
dc.format
application/pdf  
dc.language.iso
eng  
dc.publisher
Elsevier Science Inc  
dc.rights
info:eu-repo/semantics/openAccess  
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/  
dc.subject
CRYOPRESERVATION  
dc.subject
DONKEY  
dc.subject
EGG YOLK  
dc.subject
SEMEN  
dc.subject.classification
Otras Ciencias Veterinarias  
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Ciencias Veterinarias  
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CIENCIAS AGRÍCOLAS  
dc.title
Comparison of whole and centrifuged egg-yolk added to Kenney´s and Lactose-EDTA extenders for donkey semen cryopreservation  
dc.type
info:eu-repo/semantics/article  
dc.type
info:ar-repo/semantics/artículo  
dc.type
info:eu-repo/semantics/publishedVersion  
dc.date.updated
2021-05-27T12:38:39Z  
dc.journal.volume
65  
dc.journal.pagination
12-18  
dc.journal.pais
Estados Unidos  
dc.description.fil
Fil: Ferrante, Alejandro Antonio. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias. Instituto de Investigacion y Tecnología en Reproducción Animal; Argentina  
dc.description.fil
Fil: Baca Castex, Clara. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias. Instituto de Investigacion y Tecnología en Reproducción Animal; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario; Argentina  
dc.description.fil
Fil: Bruno, Santiago Rafael. No especifíca;  
dc.description.fil
Fil: Arraztoa, Claudia Cecilia. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias. Instituto de Investigacion y Tecnología en Reproducción Animal; Argentina  
dc.description.fil
Fil: Plaza, Jessica Paula. No especifíca;  
dc.description.fil
Fil: Neild, Deborah. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias. Instituto de Investigacion y Tecnología en Reproducción Animal; Argentina  
dc.description.fil
Fil: Miragaya, Marcelo. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias. Instituto de Investigacion y Tecnología en Reproducción Animal; Argentina  
dc.journal.title
Journal of Equine Veterinary Science  
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/http://linkinghub.elsevier.com/retrieve/pii/S0737080617306780  
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/https://doi.org/10.1016/j.jevs.2018.02.024