Artículo
Oxidative stress in mouse liver caused by dietary amino acid deprivation : protective effect of methionine
Ronchi, Virginia Paola
; Giudici, Ana Marcela
; Mendieta, Julieta Renee
; Caballero, Veronica Jorgelina
; Chisari, Andrea Nancy
; Sanllorenti, Pedro M.; Conde, Ruben Danilo
Fecha de publicación:
06/2010
Editorial:
Springer
Revista:
Journal Of Physiology And Biochemistry
ISSN:
1138-7548
e-ISSN:
1877-8755
Idioma:
Inglés
Tipo de recurso:
Artículo publicado
Clasificación temática:
Resumen
The aim of this work was to evaluate the effects of a diet depleted of amino acids (protein-free diet, or PFD), as well as the supplementation with methionine (PFD+Met), on the antioxidant status of the female mouse liver. With this purpose, cytosolic protein spots from two-dimensional non-equilibrium pH gel electrophoresis were identified by several procedures, such as mass spectrometry, Western blot, gel matching and enzymatic activity. PFD decreased the contents of catalase (CAT), peroxiredoxin I (Prx-I), and glutathione peroxidase (GPx) by 67%, 37% and 45%, respectively. Gene expression analyses showed that PFD caused a decrease in CAT (−20%) and GPx (−30%) mRNA levels but did not change that of Prx-I. It was also found that, when compared to a normal diet, PFD increased the liver contents of both reactive oxygen species (+50%) and oxidized protein (+88%) and decreased that of glutathione (−45%). Supplementation of PFD with Met prevented these latter effects to varying degrees, whereas CAT, Prx-I and GPx mRNA levels resulted unmodified. Present results suggest that dietary amino acid deprivation deranges the liver antioxidant defences, and this can be, in part, overcome by supplementation with Met.
Archivos asociados
Licencia
Identificadores
Colecciones
Articulos(IIB)
Articulos de INSTITUTO DE INVESTIGACIONES BIOLOGICAS
Articulos de INSTITUTO DE INVESTIGACIONES BIOLOGICAS
Citación
Ronchi, Virginia Paola; Giudici, Ana Marcela; Mendieta, Julieta Renee; Caballero, Veronica Jorgelina; Chisari, Andrea Nancy; et al.; Oxidative stress in mouse liver caused by dietary amino acid deprivation : protective effect of methionine; Springer; Journal Of Physiology And Biochemistry; 66; 2; 6-2010; 93-103
Compartir
Altmétricas