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dc.contributor.author
Campero, Lucía María  
dc.contributor.author
Schott, Franziska  
dc.contributor.author
Gottstein, Bruno  
dc.contributor.author
Deplazes, Peter  
dc.contributor.author
Sidler, Xaver  
dc.contributor.author
Basso, Walter  
dc.date.available
2021-05-14T13:47:44Z  
dc.date.issued
2020-05  
dc.identifier.citation
Campero, Lucía María; Schott, Franziska; Gottstein, Bruno; Deplazes, Peter; Sidler, Xaver; et al.; Detection of antibodies to Toxoplasma gondii in oral fluid from pigs; Elsevier; International Journal for Parasitology; 50; 5; 5-2020; 349-355  
dc.identifier.issn
0020-7519  
dc.identifier.uri
http://hdl.handle.net/11336/132078  
dc.description.abstract
Toxoplasma gondii-infected pigs play a major role as a source of infection for humans and detection of high-risk herds is essential to implement control measures at the farm level. The aim of this study was to determine whether oral fluid (OF) could be used as a matrix to detect antibodies against T. gondii in infected pigs by immunoblot (IB). For this, OF from experimentally inoculated sows (n = 8) (serial samples) and naturally exposed group-housed fatteners (n = 42 groups, one sample/group) were analysed for IgG and IgA against T. gondii-SAG1 antigen by IB. Simultaneously, each animal was serologically tested for anti-T. gondii IgG by ELISA. Specific IgG was detected in the sera of all inoculated sows from 2 to 3 weeks post inoculation (pi) and in 3.4 to 92% of the pigs in 13 out of 42 groups. Experimentally inoculated sows showed positive OF-IB results for IgA (100%) and IgG (87.5%) at 1.5 weeks pi and continued yielding positive results for IgA (87.5–75%) and IgG (50%) until 4 weeks pi; however, from 8 weeks pi the frequency of detection of both isotypes was lower, despite constantly positive IgG values in serum-ELISA. Interestingly, consecutive daily samplings for 4 days at 13 and 30 weeks pi showed inconsistent results for some sows, showing that the antibody concentration in OF is prone to timely variations. Pooled OF from groups with 91 and 92% of seropositive pigs yielded positive IB results for IgG and IgA. Fattener groups with ≤13% of seropositive pigs gave negative IB results to both isotypes. Our results showed that antibodies to T. gondii can be detected in OF from infected pigs, and that IgA seems to be a more adequate target than IgG. Although OF does not seem to be a robust matrix to assess the serological status for T. gondii in individual animals, this diagnostic approach represents an interesting non-invasive, low-cost and animal welfare friendly option as a screening method at the farm level to determine high exposure to T. gondii in the herd.  
dc.format
application/pdf  
dc.language.iso
eng  
dc.publisher
Elsevier  
dc.rights
info:eu-repo/semantics/restrictedAccess  
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/  
dc.subject
COTTON ROPE  
dc.subject
IGA  
dc.subject
IGG  
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SALIVA  
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SWINE  
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TOXOPLASMOSIS  
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Otras Ciencias Veterinarias  
dc.subject.classification
Ciencias Veterinarias  
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CIENCIAS AGRÍCOLAS  
dc.title
Detection of antibodies to Toxoplasma gondii in oral fluid from pigs  
dc.type
info:eu-repo/semantics/article  
dc.type
info:ar-repo/semantics/artículo  
dc.type
info:eu-repo/semantics/publishedVersion  
dc.date.updated
2021-04-19T14:08:34Z  
dc.journal.volume
50  
dc.journal.number
5  
dc.journal.pagination
349-355  
dc.journal.pais
Países Bajos  
dc.journal.ciudad
Amsterdam  
dc.description.fil
Fil: Campero, Lucía María. University of Bern; Suiza. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Departamento de Epizootiología y Salud Pública. Laboratorio de Inmunoparasitología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; Argentina  
dc.description.fil
Fil: Schott, Franziska. Universitat Zurich; Suiza  
dc.description.fil
Fil: Gottstein, Bruno. University of Bern; Suiza  
dc.description.fil
Fil: Deplazes, Peter. Universitat Zurich; Suiza  
dc.description.fil
Fil: Sidler, Xaver. Universitat Zurich; Suiza  
dc.description.fil
Fil: Basso, Walter. University of Bern; Suiza. Universitat Zurich; Suiza  
dc.journal.title
International Journal for Parasitology  
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1016/j.ijpara.2019.11.002  
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/abs/pii/S0020751919303029?via%3Dihub