Artículo
Relevance of angiogenesis in autoimmune testis inflammation
Gualdoni, Gisela Soledad
; Jacobo, Patricia Verónica
; Sobarzo, Cristian Marcelo; Pérez, Cecilia Valeria
; Haro Durand, Luis Alberto
; Theas, Maria Susana
; Lustig, Livia
; Guazzone, Vanesa Anabella
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Fecha de publicación:
12/2020
Editorial:
Oxford University Press
Revista:
Molecular Human Reproduction
ISSN:
1360-9947
Idioma:
Inglés
Tipo de recurso:
Artículo publicado
Clasificación temática:
Resumen
Experimental autoimmune orchitis (EAO) is a useful model to study organ-specific autoimmunity and chronic testicular inflammation. This model reflects testicular pathological changes reported in immunological infertility in men. Progression of EAO in rodents is associated with a significantly increased percentage of testicular endothelial cells and interstitial testicular blood vessels, indicating an ongoing angiogenic process. Vascular endothelial growth factor A (VEGFA), the main regulator of physiological and pathological angiogenesis, can stimulate endothelial cell proliferation, chemotaxis and vascular permeability. The aim of this study was to explore therole of VEGFA in the pathogenesis of testicular inflammation. Our results found VEGFA expression in Leydig cells, endothelial cells and macrophages in testis of rats with autoimmune orchitis. VEGFA level was significantly higher in testicular fluid and serum of rats at the end of the immunization period, preceding testicular damage. VEGF receptor (VEGFR) 1 is expressed mainly in testicular endothelial cells, whereas VEGFR2 was detected in germ cells and vascular smooth muscle cells. Both receptors were expressed in testicular interstitial cells. VEGFR2 increased after the immunization period in the testicular interstitium and VEGFR1 was downregulated in EAO testis. In-vivospecific VEGFA inhibition by Bevacizumab prevented the increase in blood vessel number and reduced EAO incidence and severity. Our results unveil relevance of VEGFA-VEGFR axis during orchitis development, suggesting that VEGFA might be an early marker of testicularinflammation and Bevacizumab a therapeutic tool for treatment of testicular inflammation associated with subfertility and infertility
Palabras clave:
ORCHITIS
,
VEGF
,
VEGFR
,
HIF
,
MALE INFERTILITY
Archivos asociados
Licencia
Identificadores
Colecciones
Articulos(IBYME)
Articulos de INST.DE BIOLOGIA Y MEDICINA EXPERIMENTAL (I)
Articulos de INST.DE BIOLOGIA Y MEDICINA EXPERIMENTAL (I)
Articulos(INBIOMED)
Articulos de INSTITUTO DE INVESTIGACIONES BIOMEDICAS
Articulos de INSTITUTO DE INVESTIGACIONES BIOMEDICAS
Citación
Gualdoni, Gisela Soledad; Jacobo, Patricia Verónica; Sobarzo, Cristian Marcelo; Pérez, Cecilia Valeria; Haro Durand, Luis Alberto; et al.; Relevance of angiogenesis in autoimmune testis inflammation; Oxford University Press; Molecular Human Reproduction; 27; 2; 12-2020; 1-14
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