Artículo
Emergence and dominance of CTX-M-15 extended spectrum beta-lactamase among Escherichia coli isolates from children
Fecha de publicación:
04/2011
Editorial:
Mary Ann Liebert Inc
Revista:
Microbial Drug Resistance-mechanisms Epidemiology And Disease
ISSN:
1076-6294
Idioma:
Inglés
Tipo de recurso:
Artículo publicado
Clasificación temática:
Resumen
Of forty-seven extended-spectrum cephalosporin-resistant Escherichia coli isolates, collected from children at the Children’s Hospital in 2006 (Tunis, Tunisia), we analyzed 32 isolates that were genotypically different by enterobacterial repetitive intergenic consensus -polymerase chain reaction. For all isolates, the double-disk diffusion test revealed synergy between clavulanate and cefotaxime and/or ceftazidime, suggesting the production of extended-spectrum beta-lactamases. Polymerase chain reaction experiments, performed on plasmid DNA, and sequencing revealed the presence of blaTEM-1B (26 isolates, 81%), blaTEM-34(IRT-6) (3 isolates, 9%), blaSHV-12 (2 isolates, 6%), and blaCTX-M-15 (31 isolates, 97%). Further, the insertion sequence ISEcp1 was found upstream from the blaCTX-M-15 gene in 11 isolates. The bla genes were found alone or in various combinations in a single isolate. blaTEM-1B and blaCTX-M-15 genes were detected in 26 out of the 32 isolates. Three isolates harbored both blaTEM-34(IRT-6) and blaCTX-M-15. blaSHV-12 was identified either alone or with blaCTX-M-15 in a single isolate. Our investigation showed the dominance of CTX-M-type extended-spectrum beta-lactamases, with CTX-M-15 particularly common, and to our best knowledge, this is the first report of the coexistence of CTX-M-15 and IRT-6 in E. coli isolates from children in Tunisia.
Palabras clave:
Microbial Drug Resistance
,
Mechanisms
,
Esbl
,
Molecular Characterization
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Identificadores
Colecciones
Articulos(OCA HOUSSAY)
Articulos de OFICINA DE COORDINACION ADMINISTRATIVA HOUSSAY
Articulos de OFICINA DE COORDINACION ADMINISTRATIVA HOUSSAY
Citación
Réjiba, Samia; Mercuri, Paola Sandra; Power, Pablo; Kechrid, Amel; Emergence and dominance of CTX-M-15 extended spectrum beta-lactamase among Escherichia coli isolates from children; Mary Ann Liebert Inc; Microbial Drug Resistance-mechanisms Epidemiology And Disease; 17; 2; 4-2011; 135-140
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