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dc.contributor.author Polizio, Ariel Héctor
dc.contributor.author Chinchilla, Pilar
dc.contributor.author Chen, Xiaole
dc.contributor.author Kim, Sangbumn
dc.contributor.author Manning, David R.
dc.contributor.author Riobo, Natalia A.
dc.date.available 2017-02-20T19:33:49Z
dc.date.issued 2011-07
dc.identifier.citation Polizio, Ariel Héctor; Chinchilla, Pilar; Chen, Xiaole; Kim, Sangbumn; Manning, David R.; et al.; Heterotrimeric Gi Proteins link Hedgehog signaling to activation of Rho small GTPases to promote fibroblast migration; American Society For Biochemistry And Molecular Biology; Journal Of Biological Chemistry; 286; 7-2011; 19589-19596
dc.identifier.issn 0021-9258
dc.identifier.uri http://hdl.handle.net/11336/13181
dc.description.abstract Evidence supporting the functionality of Smoothened (SMO), an essential transducer in most pathways engaged by Hedgehog (Hh), as a Gi-coupled receptor contrasts with the lack of an apparently consistent requirement for Gi in Hh signal transduction. In the present study, we sought to evaluate the role of SMO-Gi coupling in fibroblast migration induced by Sonic Hedgehog (Shh). Our results demonstrate an absolute requirement for Gi in Shh-induced fibroblast migration. We found that Shh acutely stimulates the small Rho GTPases Rac1 and RhoA via SMO through a Gi protein- and PI3K-dependent mechanism, and that these are required for cell migration. These responses were independent of transcription by Gli and of the C-terminal domain of SMO, as we show using a combination of molecular and genetic tools. Our findings provide a mechanistic model for fibroblast migration in response to Shh and underscore the role of SMO-Gi coupling in non-canonical Hh signaling.
dc.format application/pdf
dc.language.iso eng
dc.publisher American Society For Biochemistry And Molecular Biology
dc.rights info:eu-repo/semantics/openAccess
dc.rights.uri https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject Sonic HEDGEHOG
dc.subject migration
dc.subject cancer
dc.subject signal pathways
dc.subject.classification Bioquímica y Biología Molecular
dc.subject.classification Medicina Básica
dc.subject.classification CIENCIAS MÉDICAS Y DE LA SALUD
dc.title Heterotrimeric Gi Proteins link Hedgehog signaling to activation of Rho small GTPases to promote fibroblast migration
dc.type info:eu-repo/semantics/article
dc.type info:ar-repo/semantics/artículo
dc.type info:eu-repo/semantics/publishedVersion
dc.date.updated 2017-02-15T14:08:20Z
dc.journal.volume 286
dc.journal.pagination 19589-19596
dc.journal.pais Estados Unidos
dc.journal.ciudad Washignton
dc.description.fil Fil: Polizio, Ariel Héctor. Thomas Jefferson University; Estados Unidos. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; Argentina
dc.description.fil Fil: Chinchilla, Pilar. Thomas Jefferson University; Estados Unidos
dc.description.fil Fil: Chen, Xiaole. Thomas Jefferson University; Estados Unidos
dc.description.fil Fil: Kim, Sangbumn. Thomas Jefferson University; Estados Unidos
dc.description.fil Fil: Manning, David R.. University Of Pennsylvania; Estados Unidos
dc.description.fil Fil: Riobo, Natalia A.. Thomas Jefferson University; Estados Unidos
dc.journal.title Journal Of Biological Chemistry
dc.relation.alternativeid info:eu-repo/semantics/altIdentifier/url/http://www.jbc.org/content/286/22/19589.long
dc.relation.alternativeid info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1074/jbc.M110.197111
dc.relation.alternativeid info:eu-repo/semantics/altIdentifier/url/https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3103338/


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info:eu-repo/semantics/openAccess Excepto donde se diga explícitamente, este item se publica bajo la siguiente descripción: Creative Commons Attribution-NonCommercial-ShareAlike 2.5 Unported (CC BY-NC-SA 2.5)